Mouse Bmp4 ELISA Kit

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  • Alternative name

    Mouse Bmp-4 ELISA Kit;Mouse bone morphogenetic protein 2B ELISA Kit;Mouse BMP-2B ELISA Kit;Mouse Dvr-4 ELISA Kit;Mouse Bmp2b ELISA Kit;Mouse Bmp2b-1 ELISA Kit;Mouse Bmp2b1 ELISA Kit;Mouse bone morphogenetic protein 4 ELISA Kit;

  • Catalog
    E045925
  • species
    Mouse
  • GeneBmp4
  • Standard CurveMouse Bmp4 ELISA Kit
  • Other Species Human BMP4 ELISA KitBovine BMP4 ELISA KitChicken BMP4 ELISA KitRabbit BMP4 ELISA KitRat Bmp4 ELISA Kit
  • SpecificityNatural and recombinant mouse BMP-4
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity Sample For quantitative detection of mouse BMP-4 in bone tissue, cell culture supernates an
  • Intended UseMouse Bmp4 ELISA Kit allows for the in vitro quantitative determination of Bmp4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: Mouse BMP-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for BMP-4 has been precoated onto 96-well plates. Standards (Expression system for standard: CHO, Immunogen sequence: S293-R408) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse BMP-4 amount of sample captured in plate. Background: Bone morphogenetic protein 4 is a protein that in humans is encoded by the BMP4 gene which is located to 14q22-q23. The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. BMP4 is a polypeptide belonging to the TGF-beta superfamily of proteins. It, like other bone morphogenetic proteins, is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been shown to be involved in muscle development, bone mineralization, and uteric bud development. BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva in which it is underexpressed. In human embryonic development, BMP4 is a critical signaling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog (released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. Also, BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal (by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate.
  • Mouse Bone morphogenetic protein 4 Protein information
  • Uniprot ID BMP4_MOUSE
  • Uniprot AC P21275;
  • UniGene Mm.6813;
  • GeneID 12159
  • KEGG mmu:12159;
  • Mouse Bone morphogenetic protein 4 Protein SEQUENCE
  • SEQUENCE 408 AA; 46497 MW; 35053D844624EF9D CRC64;

    MIPGNRMLMV VLLCQVLLGG ASHASLIPET GKKKVAEIQG HAGGRRSGQS

    HELLRDFEAT LLQMFGLRRR PQPSKSAVIP DYMRDLYRLQ SGEEEEEEQS

    QGTGLEYPER PASRANTVRS FHHEEHLENI PGTSESSAFR FLFNLSSIPE

    NEVISSAELR LFREQVDQGP DWEQGFHRIN IYEVMKPPAE MVPGHLITRL

    LDTRLVHHNV TRWETFDVSP AVLRWTREKQ PNYGLAIEVT HLHQTRTHQG

    QHVRISRSLP QGSGDWAQLR PLLVTFGHDG RGHTLTRRRA KRSPKHHPQR

    SRKKNKNCRR HSLYVDFSDV GWNDWIVAPP GYQAFYCHGD CPFPLADHLN

    STNHAIVQTL VNSVNSSIPK ACCVPTELSA ISMLYLDEYD KVVLKNYQEM

    VVEGCGCR

  • UCSC uc007thd.2; mouse.;



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