Human LBP ELISA Kit

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  • Alternative name

    Human LBP ELISA Kit;Human BPIFD2 ELISA Kit;Human lipopolysaccharide binding protein ELISA Kit;Human lipopolysaccharide-binding protein ELISA Kit;Human BPI fold containing family D, member 2 ELISA Kit;Human LPS-binding protein ELISA Kit;

  • Catalog
    E022255
  • species
    Human
  • GeneLBP
  • Standard CurveHuman LBP ELISA Kit
  • Other Species Mouse Lbp ELISA KitBovine LBP ELISA KitRabbit LBP ELISA KitRat Lbp ELISA Kit
  • SpecificityNatural and recombinant human LBP
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity Sample For quantitative detection of human LBP in cell culture supernates, serum and plasma
  • Intended UseHuman LBP ELISA Kit allows for the in vitro quantitative determination of LBP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: Human LBP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for LBP has been precoated onto 96-well plates. Standards(NSO, A26-V481) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LBP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human LBP amount of sample captured in plate. Background: Lipopolysaccharide binding protein is a protein that in humans is encoded by the LBP gene. This gene is mapped to 20q11.23. LBP is a soluble acute-phase protein that binds to bacterial lipopolysaccharide(or LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4. It is present in the cerebrospinal fluid of patients with pneumococcal meningitis. The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. LBP is made in the liver during the acute phase of infections and is thought to function as a carrier for LPS and to help control LPS-dependent monocyte responses.
  • Human Lipopolysaccharide-binding protein Protein information
  • Uniprot ID LBP_HUMAN
  • Uniprot AC P18428; B2R938; O43438; Q92672; Q9H403; Q9UD66;
  • UniGene Hs.154078;
  • GeneID 3929
  • KEGG hsa:3929;
  • Human Lipopolysaccharide-binding protein Protein SEQUENCE
  • SEQUENCE 481 AA; 53384 MW; 5A0E4B9E5E604C72 CRC64;

    MGALARALPS ILLALLLTST PEALGANPGL VARITDKGLQ YAAQEGLLAL

    QSELLRITLP DFTGDLRIPH VGRGRYEFHS LNIHSCELLH SALRPVPGQG

    LSLSISDSSI RVQGRWKVRK SFFKLQGSFD VSVKGISISV NLLLGSESSG

    RPTVTASSCS SDIADVEVDM SGDLGWLLNL FHNQIESKFQ KVLESRICEM

    IQKSVSSDLQ PYLQTLPVTT EIDSFADIDY SLVEAPRATA QMLEVMFKGE

    IFHRNHRSPV TLLAAVMSLP EEHNKMVYFA ISDYVFNTAS LVYHEEGYLN

    FSITDDMIPP DSNIRLTTKS FRPFVPRLAR LYPNMNLELQ GSVPSAPLLN

    FSPGNLSVDP YMEIDAFVLL PSSSKEPVFR LSVATNVSAT LTFNTSKITG

    FLKPGKVKVE LKESKVGLFN AELLEALLNY YILNTFYPKF NDKLAEGFPL

    PLLKRVQLYD LGLQIHKDFL FLGANVQYMR V

  • UCSC uc002xic.3; human.;



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