Human LBP ELISA Kit

Alternative name
Human LBP ELISA Kit;Human BPIFD2 ELISA Kit;Human lipopolysaccharide binding protein ELISA Kit;Human lipopolysaccharide-binding protein ELISA Kit;Human BPI fold containing family D, member 2 ELISA Kit;Human LPS-binding protein ELISA Kit;
Catalog
E022255
species
Human
GeneLBP
SpecificityNatural and recombinant human LBP
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity Sample For quantitative detection of human LBP in cell culture supernates, serum and plasma
Intended UseHuman LBP ELISA Kit allows for the in vitro quantitative determination of LBP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: Human LBP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for LBP has been precoated onto 96-well plates. Standards(NSO, A26-V481) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LBP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human LBP amount of sample captured in plate. Background: Lipopolysaccharide binding protein is a protein that in humans is encoded by the LBP gene. This gene is mapped to 20q11.23. LBP is a soluble acute-phase protein that binds to bacterial lipopolysaccharide(or LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4. It is present in the cerebrospinal fluid of patients with pneumococcal meningitis. The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. LBP is made in the liver during the acute phase of infections and is thought to function as a carrier for LPS and to help control LPS-dependent monocyte responses.
Human Lipopolysaccharide-binding protein Protein information
Uniprot ID LBP_HUMAN
Uniprot AC P18428; B2R938; O43438; Q92672; Q9H403; Q9UD66;
UniGene Hs.154078;
GeneID 3929
KEGG hsa:3929;
Human Lipopolysaccharide-binding protein Protein SEQUENCE
SEQUENCE 481 AA; 53384 MW; 5A0E4B9E5E604C72 CRC64;
MGALARALPS ILLALLLTST PEALGANPGL VARITDKGLQ YAAQEGLLAL
QSELLRITLP DFTGDLRIPH VGRGRYEFHS LNIHSCELLH SALRPVPGQG
LSLSISDSSI RVQGRWKVRK SFFKLQGSFD VSVKGISISV NLLLGSESSG
RPTVTASSCS SDIADVEVDM SGDLGWLLNL FHNQIESKFQ KVLESRICEM
IQKSVSSDLQ PYLQTLPVTT EIDSFADIDY SLVEAPRATA QMLEVMFKGE
IFHRNHRSPV TLLAAVMSLP EEHNKMVYFA ISDYVFNTAS LVYHEEGYLN
FSITDDMIPP DSNIRLTTKS FRPFVPRLAR LYPNMNLELQ GSVPSAPLLN
FSPGNLSVDP YMEIDAFVLL PSSSKEPVFR LSVATNVSAT LTFNTSKITG
FLKPGKVKVE LKESKVGLFN AELLEALLNY YILNTFYPKF NDKLAEGFPL
PLLKRVQLYD LGLQIHKDFL FLGANVQYMR V
UCSC uc002xic.3; human.;

Category

Human LBP ELISA Kit

Human LBP ELISA Kit;Human BPIFD2 ELISA Kit;Human lipopolysaccharide binding protein ELISA Kit;Human lipopolysaccharide-binding protein ELISA Kit;Human BPI fold containing family D, member 2 ELISA Kit;Human LPS-binding protein ELISA Kit;