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Human PADI2 ELISA Kit

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  • Alternative name

    Human PAD-H19 ELISA Kit;Human Peptidylarginine deiminase II ELISA Kit;Human protein-arginine deiminase type II ELISA Kit;Human KIAA0994 ELISA Kit;Human PAD2 ELISA Kit;Human PDI2 ELISA Kit;Human peptidyl arginine deiminase 2 ELISA Kit;Human protein-arginine deiminase type-2 ELISA Kit;Human peptidyl arginine deiminase, type II ELISA Kit;
  • Catalog
    E028566
  • species
    Human
  • GenePADI2
  • Standard CurveHuman PADI2 ELISA Kit
  • Other Species Mouse Padi2 ELISA KitRat Padi2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Peptidyl Arginine Deiminase Type II (PADI2). No significant cross-reactivity or interference between Peptidyl Arginine Deiminase Type II (PADI2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.63ng/mL.
  • Intended UseHuman PADI2 ELISA Kit allows for the in vitro quantitative determination of PADI2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peptidyl Arginine Deiminase Type II (PADI2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peptidyl Arginine Deiminase Type II (PADI2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peptidyl Arginine Deiminase Type II (PADI2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peptidyl Arginine Deiminase Type II (PADI2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Protein-arginine deiminase type-2 Protein information
  • Uniprot ID PADI2_HUMAN
  • Uniprot AC Q9Y2J8; Q96DA7; Q9UPN2;
  • UniGene Hs.33455;
  • GeneID 11240
  • KEGG hsa:11240;
  • Human Protein-arginine deiminase type-2 Protein SEQUENCE

  • SEQUENCE 665 AA; 75564 MW; 8D417A87A02D6839 CRC64;

    MLRERTVRLQ YGSRVEAVYV LGTYLWTDVY SAAPAGAQTF SLKHSEHVWV

    EVVRDGEAEE VATNGKQRWL LSPSTTLRVT MSQASTEASS DKVTVNYYDE

    EGSIPIDQAG LFLTAIEISL DVDADRDGVV EKNNPKKASW TWGPEGQGAI

    LLVNCDRETP WLPKEDCRDE KVYSKEDLKD MSQMILRTKG PDRLPAGYEI

    VLYISMSDSD KVGVFYVENP FFGQRYIHIL GRRKLYHVVK YTGGSAELLF

    FVEGLCFPDE GFSGLVSIHV SLLEYMAQDI PLTPIFTDTV IFRIAPWIMT

    PNILPPVSVF VCCMKDNYLF LKEVKNLVEK TNCELKVCFQ YLNRGDRWIQ

    DEIEFGYIEA PHKGFPVVLD SPRDGNLKDF PVKELLGPDF GYVTREPLFE

    SVTSLDSFGN LEVSPPVTVN GKTYPLGRIL IGSSFPLSGG RRMTKVVRDF

    LKAQQVQAPV ELYSDWLTVG HVDEFMSFVP IPGTKKFLLL MASTSACYKL

    FREKQKDGHG EAIMFKGLGG MSSKRITINK ILSNESLVQE NLYFQRCLDW

    NRDILKKELG LTEQDIIDLP ALFKMDEDHR ARAFFPNMVN MIVLDKDLGI

    PKPFGPQVEE ECCLEMHVRG LLEPLGLECT FIDDISAYHK FLGEVHCGTN

    VRRKPFTFKW WHMVP

  • UCSC uc001baf.4; human. [Q9Y2J8-1];


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