Intended UseHuman IFNr ELISA Kit allows for the in vitro quantitative determination of IFNr , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IFNgamma polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IFNgamma polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IFNgamma amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IFNgamma can be calculated.
Background: Interferon-gamma (IFN-gamma), early in its history was known as immune interferon, was recognized in 1970. It dimerized soluble cytokine and is the only member of the type II class of interferons. The IFN-gamma monomer consists of a core of six alpha-helices and an extended unfolded sequence in the C-terminal region. It is produced predominantly by natural killer (NK) and natural killer T (NKT) cells. It is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Interferon-gamma 1b is used to treat chronic granulomatous disease and osteopetrosis.