Human CRYAB ELISA Kit

Alternative name
Human Alpha(B)-crystallin ELISA Kit;Human heat shock protein beta-5 ELISA Kit;Human HSPB5 ELISA Kit;Human renal carcinoma antigen NY-REN-27 ELISA Kit;Human rosenthal fiber component ELISA Kit;Human CRYA2 ELISA Kit;Human CMD1II ELISA Kit;Human CTPP2 ELISA Kit;Human CTRCT16 ELISA Kit;Human HEL-S-101 ELISA Kit;Human MFM2 ELISA Kit;Human crystallin alpha B ELISA Kit;Human alpha-crystallin B chain ELISA Kit;Human epididymis secretory protein Li 101 ELISA Kit;Human heat-shock 20 kD like-protein ELISA Kit;
Catalog
E001480
species
Human
GeneCRYAB
SpecificityThis assay has high sensitivity and excellent specificity for detection of CRYalphaB. No significant cross-reactivity or interference between CRYalphaB and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CRYalphaB and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CRYAB ELISA Kit allows for the in vitro quantitative determination of CRYAB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeurobiology
Product Description
specificalIntended Uses: This CRYalphaB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CRYalphaB. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||CRYalphaB ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CRYalphaB. Standards or samples are then added to the microtiter plate wells and CRYalphaB if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CRYalphaB present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CRYalphaB are added to each well to "sandwich" the CRYalphaB immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CRYalphaB and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CRYalphaB concentration in each sample is interpolated from this standard curve.
Human Alpha-crystallin B chain Protein information
Uniprot ID CRYAB_HUMAN
Uniprot AC P02511; B0YIX0; O43416; Q9UC37; Q9UC38; Q9UC39; Q9UC40; Q9UC41;
UniGene Hs.53454; Hs.703770;
GeneID 1410
KEGG hsa:1410;
Human Alpha-crystallin B chain Protein SEQUENCE
SEQUENCE 175 AA; 20159 MW; AE08BED46B7849CB CRC64;
MDIAIHHPWI RRPFFPFHSP SRLFDQFFGE HLLESDLFPT STSLSPFYLR
PPSFLRAPSW FDTGLSEMRL EKDRFSVNLD VKHFSPEELK VKVLGDVIEV
HGKHEERQDE HGFISREFHR KYRIPADVDP LTITSSLSSD GVLTVNGPRK
QVSGPERTIP ITREEKPAVT AAPKK

Human CRYAB ELISA Kit

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