SpecificityThis assay has high sensitivity and excellent specificity for detection of CRYalphaB. No significant cross-reactivity or interference between CRYalphaB and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CRYalphaB and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseHuman CRYAB ELISA Kit allows for the in vitro quantitative determination of CRYAB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalIntended Uses: This CRYalphaB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CRYalphaB. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay||CRYalphaB ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CRYalphaB. Standards or samples are then added to the microtiter plate wells and CRYalphaB if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CRYalphaB present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CRYalphaB are added to each well to "sandwich" the CRYalphaB immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CRYalphaB and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CRYalphaB concentration in each sample is interpolated from this standard curve.
Human Alpha-crystallin B chain Protein information
P02511; B0YIX0; O43416; Q9UC37; Q9UC38; Q9UC39; Q9UC40; Q9UC41;