Human EIF2S1 ELISA Kit

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  • Alternative name

    Human eukaryotic translation initiation factor 2 subunit alpha ELISA Kit;Human eIF-2-alpha ELISA Kit;Human EIF-2A ELISA Kit;Human EIF-2alpha ELISA Kit;Human EIF2A ELISA Kit;Human EIF-2 ELISA Kit;Human EIF2 ELISA Kit;Human eukaryotic translation initiation factor 2 subunit 1 ELISA Kit;Human eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa ELISA Kit;

  • Catalog
    E013728
  • species
    Human
  • GeneEIF2S1
  • Standard CurveHuman EIF2S1 ELISA Kit
  • Other Species Mouse Eif2s1 ELISA KitBovine EIF2S1 ELISA KitChicken EIF2S1 ELISA KitPorcine EIF2S1 ELISA KitRabbit EIF2S1 ELISA KitRat Eif2s1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of EIF2S1. No significant cross-reactivity or interference between EIF2S1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between EIF2S1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman EIF2S1 ELISA Kit allows for the in vitro quantitative determination of EIF2S1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical
    Intended Uses: This EIF2S1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human EIF2S1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||EIF2S1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-EIF2S1 antibody and an EIF2S1-HRP conjugate. The assay sample and buffer are incubated together with EIF2S1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EIF2S1 concentration since EIF2S1 from samples and EIF2S1-HRP conjugate compete for the anti-EIF2S1 antibody binding site. Since the number of sites is limited, as more sites are occupied by EIF2S1 from the sample, fewer sites are left to bind EIF2S1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EIF2S1 concentration in each sample is interpolated from this standard curve.
  • Human Eukaryotic translation initiation factor 2 subunit 1 Protein information
  • Uniprot ID IF2A_HUMAN
  • Uniprot AC P05198;
  • UniGene Hs.151777;
  • GeneID 1965
  • KEGG hsa:1965;
  • Human Eukaryotic translation initiation factor 2 subunit 1 Protein SEQUENCE
  • SEQUENCE 315 AA; 36112 MW; FF3E75E3816E6B1E CRC64;

    MPGLSCRFYQ HKFPEVEDVV MVNVRSIAEM GAYVSLLEYN NIEGMILLSE

    LSRRRIRSIN KLIRIGRNEC VVVIRVDKEK GYIDLSKRRV SPEEAIKCED

    KFTKSKTVYS ILRHVAEVLE YTKDEQLESL FQRTAWVFDD KYKRPGYGAY

    DAFKHAVSDP SILDSLDLNE DEREVLINNI NRRLTPQAVK IRADIEVACY

    GYEGIDAVKE ALRAGLNCST ENMPIKINLI APPRYVMTTT TLERTEGLSV

    LSQAMAVIKE KIEEKRGVFN VQMEPKVVTD TDETELARQM ERLERENAEV

    DGDDDAEEME AKAED



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