SpecificityTPAcyk measures key epitopes of TPA (Tissue Polypeptide Antigen) fragments. The monoclonal 6D7 and 3F3 antibodies used in the test are specific for cytokeratin 8 and 18, with no detectable cross reactivity to other tumor associated antigens that may be present in patient samples.
SensitivityThe minimal detectable concentration in TPAcyk ELISA is 0.1 ng/ml, defined as the concentration of TPA that corresponds to the absorbance being three standard deviations from the absorbance of standard 0 ng/ml.
Intended UseHuman TPAcyk ELISA Kit allows for the in vitro quantitative determination of TPAcyk , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Categories/FamilyOncology; Research Assays; Assay Categories
Product Description specificalPrinciple of the assay: TPAcyk ELISA is a solid phase sandwich assay based on immunochemical technique. Standards, controls and samples react simultaneously with solid phase catcher antibodies (6D7 and 3F3) and the HRP conjugated detector antibody during incubation in the microstrip wells. After washing, the TMB substrate is added. Subsequently the reaction is stopped and the absorbance is read. The developed color is directly proportional to the concentration of the analyte.