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  • Alternative name

    Human CPI-B ELISA Kit;Human liver thiol proteinase inhibitor ELISA Kit;Human Stefin-B ELISA Kit;Human CST6 ELISA Kit;Human STFB ELISA Kit;Human EPM1 ELISA Kit;Human EPM1A ELISA Kit;Human PME ELISA Kit;Human ULD ELISA Kit;Human cystatin B ELISA Kit;Human cystatin-B ELISA Kit;Human cystatin B (stefin B) ELISA Kit;

  • Catalog
  • species
  • GeneCSTB
  • Standard CurveHuman CSTB ELISA Kit
  • Other Species Mouse Cstb ELISA KitBovine CSTB ELISA KitPorcine CSTB ELISA KitRat Cstb ELISA KitSheep CSTB ELISA Kit
  • SpecificityNatural and recombinant human Cystatin B
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 10pg/ml
  • Intended UseHuman CSTB ELISA Kit allows for the in vitro quantitative determination of CSTB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    Principle of the assay: human Cystatin B ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Cystatin B has been precoated onto 96-well plates. Standards(E.coli, M2-F98) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cystatin B is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Cystatin B amount of sample captured in plate. Background: Cystatin B(CSTB), also called STFB, is a small protein that is a member of the superfamily of cysteine protease inhibitors. It has been isolated from human spleen and liver and its amino acid sequence has been fully determined. The cystatin B gene is located on 21q22.3. It is widely distributed and is localized mostly intracellularly, but has been found extracellularly. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. Its role is thought to be as a protector against the proteinases leaking from lysosomes. A cystatin B multiprotein complex might have a specific cerebellar function, and that the loss of this function might contribute to the etiopathogenesis of EPM1. Upon differentiation to myotubes, CSTB becomes excluded from the nucleus and lysosomes, suggesting that the subcellular distribution of CSTB is dependent on the differentiation status of the cell.
  • Human Cystatin-B Protein information
  • Uniprot ID CYTB_HUMAN
  • Uniprot AC P04080;
  • UniGene Hs.695;
  • GeneID 1476
  • KEGG hsa:1476;
  • Human Cystatin-B Protein SEQUENCE
  • SEQUENCE 98 AA; 11140 MW; B8076220E19D0483 CRC64;



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