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Human MYLK ELISA Kit

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  • Alternative name

    Human MLCK ELISA Kit;Human smMLCK ELISA Kit;Human kinase-related protein ELISA Kit;Human KRP ELISA Kit;Human telokin ELISA Kit;Human MLCK1 ELISA Kit;Human MYLK1 ELISA Kit;Human AAT7 ELISA Kit;Human MLCK108 ELISA Kit;Human MLCK210 ELISA Kit;Human MSTP083 ELISA Kit;Human myosin light chain kinase ELISA Kit;Human myosin light chain kinase, smooth muscle ELISA Kit;Human myosin, light polypeptide kinase ELISA Kit;Human smooth muscle myosin light chain kinase ELISA Kit;
  • Catalog
    E025475
  • species
    Human
  • GeneMYLK
  • Other Species Human MLCK/MYLK ELISA KitMouse Mylk ELISA KitMouse MLCK/MYLK ELISA KitBovine MYLK ELISA KitChicken Mylk ELISA KitPorcine MYLK ELISA KitRabbit MYLK ELISA KitSheep MYLK ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human MYLK. No significant cross-reactivity or interference between Human MYLK and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.195 ng/ml
  • Intended UseHuman MYLK ELISA Kit allows for the in vitro quantitative determination of MYLK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Introduction: Myosin-light-chain kinase also known as MYLK or MLCK is a serine/threonine-specific protein kinase that phosphorylates the regulatory light chain of myosin II. These enzymes are important in the mechanism of contraction in muscle. Once there is an influx of calcium cations (Ca++) into the muscle, either from the sarcoplasmic reticulum or, more importantly, from the extracellular space, contraction of smooth muscle fibers may begin. First, the calcium will bind to calmodulin. This binding will activate MLCK, which will go on to phosphorylate the myosin light chain at serine residue 19. This will enable the myosin crossbridge to bind to the actin filament and allow contraction to begin (through the crossbridge cycle). Since smooth muscle does not contain a troponin complex like striated muscle does, this mechanism is the main pathway for regulating smooth muscle contraction. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MLCK. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for MLCK and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain MLCK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of MLCK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Myosin light chain kinase, smooth muscle, deglutamylated form Protein information
  • Uniprot ID MYLK_HUMAN
  • Uniprot AC Q9UBG5; Q9UBY6; Q9UIT9;
  • UniGene Hs.477375;
  • GeneID 4638
  • KEGG hsa:4638;
  • Human Myosin light chain kinase, smooth muscle, deglutamylated form Protein SEQUENCE

  • SEQUENCE 1914 AA; 210715 MW; 2D094E161CE2D4BA CRC64;

    MGDVKLVASS HISKTSLSVD PSRVDSMPLT EAPAFILPPR NLCIKEGATA

    KFEGRVRGYP EPQVTWHRNG QPITSGGRFL LDCGIRGTFS LVIHAVHEED

    RGKYTCEATN GSGARQVTVE LTVEGSFAKQ LGQPVVSKTL GDRFSAPAVE

    TRPSIWGECP PKFATKLGRV VVKEGQMGRF SCKITGRPQP QVTWLKGNVP

    LQPSARVSVS EKNGMQVLEI HGVNQDDVGV YTCLVVNGSG KASMSAELSI

    QGLDSANRSF VRETKATNSD VRKEVTNVIS KESKLDSLEA AAKSKNCSSP

    QRGGSPPWAA NSQPQPPRES KLESCKDSPR TAPQTPVLQK TSSSITLQAA

    RVQPEPRAPG LGVLSPSGEE RKRPAPPRPA TFPTRQPGLG SQDVVSKAAN

    RRIPMEGQRD SAFPKFESKP QSQEVKENQT VKFRCEVSGI PKPEVAWFLE

    GTPVRRQEGS IEVYEDAGSH YLCLLKARTR DSGTYSCTAS NAQGQLSCSW

    TLQVERLAVM EVAPSFSSVL KDCAVIEGQD FVLQCSVRGT PVPRITWLLN

    GQPIQYARST CEAGVAELHI QDALPEDHGT YTCLAENALG QVSCSAWVTV

    HEKKSSRKSE YLLPVAPSKP TAPIFLQGLS DLKVMDGSQV TMTVQVSGNP

    PPEVIWLHNG NEIQESEDFH FEQRGTQHSL CIQEVFPEDT GTYTCEAWNS

    AGEVRTQAVL TVQEPHDGTQ PWFISKPRSV TASLGQSVLI SCAIAGDPFP

    TVHWLRDGKA LCKDTGHFEV LQNEDVFTLV LKKVQPWHAG QYEILLKNRV

    GECSCQVSLM LQNSSARALP RGREPASCED LCGGGVGADG GGSDRYGSLR

    PGWPARGQGW LEEEDGEDVR GVLKRRVETR QHTEEAIRQQ EVEQLDFRDL

    LGKKVSTKTL SEDDLKEIPA EQMDFRANLQ RQVKPKTVSE EERKVHSPQQ

    VDFRSVLAKK GTSKTPVPEK VPPPKPATPD FRSVLGGKKK LPAENGSSSA

    ETLNAKAVES SKPLSNAQPS GPLKPVGNAK PAETLKPMGN AKPAETLKPM

    GNAKPDENLK SASKEELKKD VKNDVNCKRG HAGTTDNEKR SESQGTAPAF

    KQKLQDVHVA EGKKLLLQCQ VSSDPPATII WTLNGKTLKT TKFIILSQEG

    SLCSVSIEKA LPEDRGLYKC VAKNDAGQAE CSCQVTVDDA PASENTKAPE

    MKSRRPKSSL PPVLGTESDA TVKKKPAPKT PPKAAMPPQI IQFPEDQKVR

    AGESVELFGK VTGTQPITCT WMKFRKQIQE SEHMKVENSE NGSKLTILAA

    RQEHCGCYTL LVENKLGSRQ AQVNLTVVDK PDPPAGTPCA SDIRSSSLTL

    SWYGSSYDGG SAVQSYSIEI WDSANKTWKE LATCRSTSFN VQDLLPDHEY

    KFRVRAINVY GTSEPSQESE LTTVGEKPEE PKDEVEVSDD DEKEPEVDYR

    TVTINTEQKV SDFYDIEERL GSGKFGQVFR LVEKKTRKVW AGKFFKAYSA

    KEKENIRQEI SIMNCLHHPK LVQCVDAFEE KANIVMVLEI VSGGELFERI

    IDEDFELTER ECIKYMRQIS EGVEYIHKQG IVHLDLKPEN IMCVNKTGTR

    IKLIDFGLAR RLENAGSLKV LFGTPEFVAP EVINYEPIGY ATDMWSIGVI

    CYILVSGLSP FMGDNDNETL ANVTSATWDF DDEAFDEISD DAKDFISNLL

    KKDMKNRLDC TQCLQHPWLM KDTKNMEAKK LSKDRMKKYM ARRKWQKTGN

    AVRAIGRLSS MAMISGLSGR KSSTGSPTSP LNAEKLESEE DVSQAFLEAV

    AEEKPHVKPY FSKTIRDLEV VEGSAARFDC KIEGYPDPEV VWFKDDQSIR

    ESRHFQIDYD EDGNCSLIIS DVCGDDDAKY TCKAVNSLGE ATCTAELIVE

    TMEEGEGEGE EEEE

  • UCSC uc003egl.4; human. [Q15746-1];


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