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Alternative name
ATG1, FLJ38455, FLJ46475, KIAA0722, ULK1, Unc 51 like kinase 1, UNC51, Unc51.1 antibody
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Catalog
A009251
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman
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Applications Tested/SuitableELISA,WB
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PurificationImmunogen affinity purified
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Immunogenunc-51-like kinase 1 (C. elegans)
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IHCImmunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using A009251( ULK1 Antibody) at dilution of 1:200
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Western Blotrat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with A009251( ULK1 Antibody) at dilution of 1:300
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Recommended dilutionWB : 1:200-1:1000;IHC:1：50-1：500
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Product Description specificalSerine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3- kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165).
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Gene nameULK1
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locationCytoplasm
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Uniprot IDO75385
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Calculated M.W.113kDa,Observed 160kD
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Observed M.W.150 kDa
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