Human CCL2 ELISA Kit

Contact Us
  • Alternative name

    Human HC11 ELISA Kit;Human Monocyte chemoattractant protein 1 ELISA Kit;Human monocyte chemotactic and activating factor ELISA Kit;Human MCAF ELISA Kit;Human monocyte chemotactic protein 1 ELISA Kit;Human MCP-1 ELISA Kit;Human monocyte secretory protein JE ELISA Kit;Human small-inducible cytokine A2 ELISA Kit;Human MCP1 ELISA Kit;Human SCYA2 ELISA Kit;Human GDCF-2 ELISA Kit;Human HSMCR30 ELISA Kit;Human SMC-CF ELISA Kit;Human C-C motif chemokine ligand 2 ELISA Kit;Human C-C motif chemokine 2 ELISA Kit;Human chemokine (C-C motif) ligand 2 ELISA Kit;Human monocyte chemoattractant protein-1 ELISA Kit;Human small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je) ELISA Kit;Human small inducible cytokine subfamily A (Cys-Cys), member 2 ELISA Kit;

  • Catalog
    E007960
  • species
    Human
  • GeneCCL2
  • Standard CurveHuman CCL2 ELISA Kit
  • Other Species Human CCL2/MCP-1 ELISA KitHuman MCP-1 / CCL2 ELISA KitMouse Ccl2 ELISA KitMouse MCP-1 / CCL2 ELISA KitMouse MCP-1/CCL2/MCAF ELISA KitBovine CCL2 ELISA KitCanine CCL2 ELISA KitGuinea Pig CCL2 ELISA KitHorse CCL2 ELISA KitMonkey CCL2 ELISA KitPorcine CCL2 ELISA KitRabbit CCL2 ELISA KitRat Ccl2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human CCL2. No significant cross-reactivity or interference between Human CCL2 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity30.184pg/ml
  • Intended UseHuman CCL2 ELISA Kit allows for the in vitro quantitative determination of CCL2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Introduction: Chemokine (C-C motif) ligand 2 (MCP-1) is a small cytokine belonging to the CC chemokine family that is also known as monocyte chemotactic protein-1 (MCP-1). MCP-1 recruits monocytes, memory T cells, and dendritic cells to sites of tissue injury and infection. This chemokine is produced as a protein precursor containing signal peptide of 23 amino acids and a mature peptide of 76 amino acids. It is a monomeric polypeptide, with a molecular weight of approximately 13kDa. As with many other CC chemokines, MCP-1 is located on chromosome 17 in humans. The cell surface receptors that bind MCP-1 are CCR2 and CCR4. It is found at the site of tooth eruption and bone degradation. In the bone, MCP-1 is expressed by mature osteoclasts and osteoblasts and is under the control of nuclear factor kappaB (NFkappaB). In human osteoclasts, it has been shown that MCP-1 and RANTES (regulated on activation normal T cell expressed and secreted) are unregulated by RANKL (receptor activator of NFkappaB ligand). Both MCP-1 and RANTES were also shown to induce the formation of TRAP-positive, multinuclear cells from M-CSF-treated monocytes in the absence of RANKL, but produced osteoclasts that lacked cathepsin K expression and resorptive capacity. It is proposed that MCP-1 and RANTES act as autocrine loop in human osteoclast differentiation. MCP-1 causes the degranulation of basophils and mast cells, an effect potentiated by pre-treatment with IL-3 and other cytokines. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MCP-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MCP-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain MCP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of MCP-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human C-C motif chemokine 2 Protein information
  • Uniprot ID CCL2_HUMAN
  • Uniprot AC P13500; B2R4V3; Q9UDF3;
  • UniGene Hs.303649;
  • GeneID 6347
  • KEGG hsa:6347;
  • Human C-C motif chemokine 2 Protein SEQUENCE
  • SEQUENCE 99 AA; 11025 MW; 45EC72361435302F CRC64;

    MKVSAALLCL LLIAATFIPQ GLAQPDAINA PVTCCYNFTN RKISVQRLAS

    YRRITSSKCP KEAVIFKTIV AKEICADPKQ KWVQDSMDHL DKQTQTPKT

  • UCSC uc002hhy.4; human.;




tag

Inquiry About Human CCL2 ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!