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Human CD70 ELISA Kit

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  • Alternative name

    Human CD27 ligand ELISA Kit;Human CD27-L ELISA Kit;Human tumor necrosis factor ligand superfamily member 7 ELISA Kit;Human CD27L ELISA Kit;Human CD27LG ELISA Kit;Human TNFSF7 ELISA Kit;Human TNLG8A ELISA Kit;Human CD70 molecule ELISA Kit;Human CD70 antigen ELISA Kit;Human Ki-24 antigen ELISA Kit;Human surface antigen CD70 ELISA Kit;Human tumor necrosis factor ligand 8A ELISA Kit;
  • Catalog
    E007522
  • species
    Human
  • GeneCD70
  • Standard CurveHuman CD70 ELISA Kit
  • Other Species Mouse Cd70 ELISA KitPorcine CD70 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CD70. No significant cross-reactivity or interference between CD70 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CD70 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CD70 ELISA Kit allows for the in vitro quantitative determination of CD70 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyImmunology
  • Product Description
    specifical    Intended Uses: This CD70 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CD70. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||CD70 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CD70 antibody and an CD70-HRP conjugate. The assay sample and buffer are incubated together with CD70-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD70 concentration since CD70 from samples and CD70-HRP conjugate compete for the anti-CD70 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD70 from the sample, fewer sites are left to bind CD70-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD70 concentration in each sample is interpolated from this standard curve.
  • Human CD70 antigen Protein information
  • Uniprot ID CD70_HUMAN
  • Uniprot AC P32970; B4DPR8; Q53XX4; Q96J57;
  • UniGene Hs.501497; Hs.715224;
  • GeneID 970
  • KEGG hsa:970;
  • Human CD70 antigen Protein SEQUENCE

  • SEQUENCE 193 AA; 21118 MW; 9265856E33BE4D50 CRC64;

    MPEEGSGCSV RRRPYGCVLR AALVPLVAGL VICLVVCIQR FAQAQQQLPL

    ESLGWDVAEL QLNHTGPQQD PRLYWQGGPA LGRSFLHGPE LDKGQLRIHR

    DGIYMVHIQV TLAICSSTTA SRHHPTTLAV GICSPASRSI SLLRLSFHQG

    CTIASQRLTP LARGDTLCTN LTGTLLPSRN TDETFFGVQW VRP

  • UCSC uc002mfi.3; human. [P32970-1];



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