Human CD40L ELISA Kit

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  • Alternative name

    CD40 ligand ELISA KIT; T-cell antigen Gp39 ELISA KIT; TNF-related activation protein ELISA KIT; TRAP ELISA KIT; Tumor necrosis factor ligand superfamily member 5 ELISA KIT; CD_antigen: CD154Cleaved into the following 2 chains:CD40 ligand, membrane form ELISA KIT; CD40 ligand, soluble formCD40LG ELISA KIT; CD40L ELISA KIT; TNFSF5 ELISA KIT; TRAP ELISA KIT; CD40-L ELISA KIT; TRAP ELISA KIT

  • Catalog
    E007256
  • species
    Human
  • GeneCD40L
  • Other Species Human CD40L/TNFSF5 ELISA KitMouse CD40L ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CD40L ELISA Kit allows for the in vitro quantitative determination of CD40L , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyImmunology
  • Product Description
    specifical
    Principle of the Assay: CD40L ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CD40L antibody and an CD40L-HRP conjugate. The assay sample and buffer are incubated together with CD40L-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD40L concentration since CD40L from samples and CD40L-HRP conjugate compete for the anti-CD40L antibody binding site. Since the number of sites is limited, as more sites are occupied by CD40L from the sample, fewer sites are left to bind CD40L-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD40L concentration in each sample is interpolated from this standard curve.



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