Human SPINT2 ELISA Kit

Alternative name
Human hepatocyte growth factor activator inhibitor type 2 ELISA Kit;Human HAI-2 ELISA Kit;Human placental bikunin ELISA Kit;Human HAI2 ELISA Kit;Human Kop ELISA Kit;Human DIAR3 ELISA Kit;Human PB ELISA Kit;Human serine peptidase inhibitor, Kunitz type 2 ELISA Kit;Human kunitz-type protease inhibitor 2 ELISA Kit;Human serine protease inhibitor, Kunitz type, 2 ELISA Kit;Human testicular tissue protein Li 183 ELISA Kit;
Catalog
E017843
species
Human
GeneSPINT2
SpecificityNatural and recombinant Human HAI-2/SPINT2
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity Intra-assay Precision Intra-Assay Precision (Precision within an assay) Three samples of kn
Intended UseHuman SPINT2 ELISA Kit allows for the in vitro quantitative determination of SPINT2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: Human HAI-2/SPINT2 ELISA Kit was based on standard sandwich enzyme-linked immunesorbent assay technology. A monoclonal antibody from mouse specific for HAI-2/SPINT2 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: M1-K197) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for HAI-2/SPINT2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human HAI-2/SPINT2 amount of sample captured in plate. Background/Introduction: Kunitz-type protease inhibitor 2 is an enzyme inhibitor that in humans is encoded by the SPINT2 gene. This gene encodes a transmembrane protein with two extracellular Kunitz domains that inhibits a variety of serine proteases. The protein inhibits HGF activator which prevents the formation of active hepatocyte growth factor. And this gene is a putative tumor suppressor, and mutations in this gene result in congenital sodium diarrhea. Multiple transcript variants encoding different isoforms have been found for this gene.
Human Kunitz-type protease inhibitor 2 Protein information
Uniprot ID SPIT2_HUMAN
Uniprot AC O43291; A8K667; B4DLU1; O00271; O14895; Q5TZQ3; Q969E0;
UniGene Hs.31439;
GeneID 10653
KEGG hsa:10653;
Human Kunitz-type protease inhibitor 2 Protein SEQUENCE
SEQUENCE 252 AA; 28228 MW; A7D3360C0EECAB2B CRC64;
MAQLCGLRRS RAFLALLGSL LLSGVLAADR ERSIHDFCLV SKVVGRCRAS
MPRWWYNVTD GSCQLFVYGG CDGNSNNYLT KEECLKKCAT VTENATGDLA
TSRNAADSSV PSAPRRQDSE DHSSDMFNYE EYCTANAVTG PCRASFPRWY
FDVERNSCNN FIYGGCRGNK NSYRSEEACM LRCFRQQENP PLPLGSKVVV
LAGLFVMVLI LFLGASMVYL IRVARRNQER ALRTVWSSGD DKEQLVKNTY
VL
UCSC uc002ohr.2; human. [O43291-1];

Human SPINT2 ELISA Kit

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