Human SPINT2 ELISA Kit

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  • Alternative name

    Human hepatocyte growth factor activator inhibitor type 2 ELISA Kit;Human HAI-2 ELISA Kit;Human placental bikunin ELISA Kit;Human HAI2 ELISA Kit;Human Kop ELISA Kit;Human DIAR3 ELISA Kit;Human PB ELISA Kit;Human serine peptidase inhibitor, Kunitz type 2 ELISA Kit;Human kunitz-type protease inhibitor 2 ELISA Kit;Human serine protease inhibitor, Kunitz type, 2 ELISA Kit;Human testicular tissue protein Li 183 ELISA Kit;

  • Catalog
    E017843
  • species
    Human
  • GeneSPINT2
  • Standard CurveHuman SPINT2 ELISA Kit
  • Other Species Mouse Spint2 ELISA Kit
  • SpecificityNatural and recombinant Human HAI-2/SPINT2
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity Intra-assay Precision Intra-Assay Precision (Precision within an assay) Three samples of kn
  • Intended UseHuman SPINT2 ELISA Kit allows for the in vitro quantitative determination of SPINT2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: Human HAI-2/SPINT2 ELISA Kit was based on standard sandwich enzyme-linked immunesorbent assay technology. A monoclonal antibody from mouse specific for HAI-2/SPINT2 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: M1-K197) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for HAI-2/SPINT2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human HAI-2/SPINT2 amount of sample captured in plate. Background/Introduction: Kunitz-type protease inhibitor 2 is an enzyme inhibitor that in humans is encoded by the SPINT2 gene. This gene encodes a transmembrane protein with two extracellular Kunitz domains that inhibits a variety of serine proteases. The protein inhibits HGF activator which prevents the formation of active hepatocyte growth factor. And this gene is a putative tumor suppressor, and mutations in this gene result in congenital sodium diarrhea. Multiple transcript variants encoding different isoforms have been found for this gene.
  • Human Kunitz-type protease inhibitor 2 Protein information
  • Uniprot ID SPIT2_HUMAN
  • Uniprot AC O43291; A8K667; B4DLU1; O00271; O14895; Q5TZQ3; Q969E0;
  • UniGene Hs.31439;
  • GeneID 10653
  • KEGG hsa:10653;
  • Human Kunitz-type protease inhibitor 2 Protein SEQUENCE
  • SEQUENCE 252 AA; 28228 MW; A7D3360C0EECAB2B CRC64;

    MAQLCGLRRS RAFLALLGSL LLSGVLAADR ERSIHDFCLV SKVVGRCRAS

    MPRWWYNVTD GSCQLFVYGG CDGNSNNYLT KEECLKKCAT VTENATGDLA

    TSRNAADSSV PSAPRRQDSE DHSSDMFNYE EYCTANAVTG PCRASFPRWY

    FDVERNSCNN FIYGGCRGNK NSYRSEEACM LRCFRQQENP PLPLGSKVVV

    LAGLFVMVLI LFLGASMVYL IRVARRNQER ALRTVWSSGD DKEQLVKNTY

    VL

  • UCSC uc002ohr.2; human. [O43291-1];



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