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Human AP2B1 ELISA Kit

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  • Alternative name

    Human AP105B ELISA Kit;Human adaptor protein complex AP-2 subunit beta ELISA Kit;Human adaptor-related protein complex 2 subunit beta ELISA Kit;Human beta-2-adaptin ELISA Kit;Human beta-adaptin ELISA Kit;Human clathrin assembly protein complex 2 beta large chain ELISA Kit;Human plasma membrane adaptor HA2/AP2 adaptin beta subunit ELISA Kit;Human ADTB2 ELISA Kit;Human CLAPB1 ELISA Kit;Human AP2-BETA ELISA Kit;Human adaptor related protein complex 2 beta 1 subunit ELISA Kit;Human AP-2 complex subunit beta ELISA Kit;Human adapter-related protein complex 2 beta subunit ELISA Kit;Human adapter-related protein complex 2 subunit beta ELISA Kit;Human adaptin, beta 2 (beta) ELISA Kit;Human clathrin-associated/assembly/adaptor protein, large, beta 1 ELISA Kit;Human testicular tissue protein Li 22 ELISA Kit;
  • Catalog
    E003186
  • species
    Human
  • GeneAP2B1
  • Other Species Mouse Ap2b1 ELISA KitBovine AP2B1 ELISA KitRat Ap2b1 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.188ng/ml
  • Intended UseHuman AP2B1 ELISA Kit allows for the in vitro quantitative determination of AP2B1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-AP2B1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-AP2B1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the AP2B1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of AP2B1 can be calculated.
  • Human AP-2 complex subunit beta Protein information
  • Uniprot ID AP2B1_HUMAN
  • Uniprot AC P63010; A6NJP3; P21851; Q7Z451; Q96J19;
  • UniGene Hs.514819;
  • GeneID 163
  • KEGG hsa:163;
  • Human AP-2 complex subunit beta Protein SEQUENCE

  • SEQUENCE 937 AA; 104553 MW; B472EE5B2AE176DF CRC64;

    MTDSKYFTTN KKGEIFELKA ELNNEKKEKR KEAVKKVIAA MTVGKDVSSL

    FPDVVNCMQT DNLELKKLVY LYLMNYAKSQ PDMAIMAVNS FVKDCEDPNP

    LIRALAVRTM GCIRVDKITE YLCEPLRKCL KDEDPYVRKT AAVCVAKLHD

    INAQMVEDQG FLDSLRDLIA DSNPMVVANA VAALSEISES HPNSNLLDLN

    PQNINKLLTA LNECTEWGQI FILDCLSNYN PKDDREAQSI CERVTPRLSH

    ANSAVVLSAV KVLMKFLELL PKDSDYYNML LKKLAPPLVT LLSGEPEVQY

    VALRNINLIV QKRPEILKQE IKVFFVKYND PIYVKLEKLD IMIRLASQAN

    IAQVLAELKE YATEVDVDFV RKAVRAIGRC AIKVEQSAER CVSTLLDLIQ

    TKVNYVVQEA IVVIRDIFRK YPNKYESIIA TLCENLDSLD EPDARAAMIW

    IVGEYAERID NADELLESFL EGFHDESTQV QLTLLTAIVK LFLKKPSETQ

    ELVQQVLSLA TQDSDNPDLR DRGYIYWRLL STDPVTAKEV VLSEKPLISE

    ETDLIEPTLL DELICHIGSL ASVYHKPPNA FVEGSHGIHR KHLPIHHGST

    DAGDSPVGTT TATNLEQPQV IPSQGDLLGD LLNLDLGPPV NVPQVSSMQM

    GAVDLLGGGL DSLVGQSFIP SSVPATFAPS PTPAVVSSGL NDLFELSTGI

    GMAPGGYVAP KAVWLPAVKA KGLEISGTFT HRQGHIYMEM NFTNKALQHM

    TDFAIQFNKN SFGVIPSTPL AIHTPLMPNQ SIDVSLPLNT LGPVMKMEPL

    NNLQVAVKNN IDVFYFSCLI PLNVLFVEDG KMERQVFLAT WKDIPNENEL

    QFQIKECHLN ADTVSSKLQN NNVYTIAKRN VEGQDMLYQS LKLTNGIWIL

    AELRIQPGNP NYTLSLKCRA PEVSQYIYQV YDSILKN

  • UCSC uc002hjq.4; human. [P63010-1];


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