Human LpPLA2 ELISA Kit

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  • Alternative name

    Phospholipase A2, membrane associated ELISA KIT; GIIC sPLA2 ELISA KIT; Group IIA phospholipase A2 ELISA KIT; Non-pancreatic secretory phospholipase A2 ELISA KIT; NPS-PLA2 ELISA KIT; Phosphatidylcholine 2-acylhydrolase 2APLA2G2A ELISA KIT; PLA2B ELISA KIT; PLA2L ELISA KIT; RASF-A ELISA KIT; NPS-PLA2 ELISA KIT

  • Catalog
    E029193
  • species
    Human
  • GeneLpPLA2
  • Standard CurveHuman LpPLA2 ELISA Kit
  • Other Species Mouse LpPLA2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Phospholipase A2, Lipoprotein Associated (LpPLA2). No significant cross-reactivity or interference between Phospholipase A2, Lipoprotein Associated (LpPLA2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.286ng/mL.
  • Intended UseHuman LpPLA2 ELISA Kit allows for the in vitro quantitative determination of LpPLA2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, Lipoprotein Associated (LpPLA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2, Lipoprotein Associated (LpPLA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2, Lipoprotein Associated (LpPLA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase A2, Lipoprotein Associated (LpPLA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.


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