Human CCL28 ELISA Kit

Alternative name
Human mucosae-associated epithelial chemokine ELISA Kit;Human MEC ELISA Kit;Human Protein CCK1 ELISA Kit;Human Small-inducible cytokine A28 ELISA Kit;Human SCYA28 ELISA Kit;Human CCK1 ELISA Kit;Human C-C motif chemokine ligand 28 ELISA Kit;Human C-C motif chemokine 28 ELISA Kit;Human CC chemokine CCL28 ELISA Kit;Human chemokine (C-C motif) ligand 28 splice variant chi ELISA Kit;Human small inducible cytokine A28 ELISA Kit;Human small inducible cytokine subfamily A (Cys-Cys), member 28 ELISA Kit;
Catalog
E007214
species
Human
GeneCCL28
SpecificityNatural and recombinant human CCL28
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity Sample For quantitative detection of human CCL28 in cell culture supernates, serum and plas
Intended UseHuman CCL28 ELISA Kit allows for the in vitro quantitative determination of CCL28 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: Human CCL28 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL28 has been precoated onto 96-well plates. Standards(Expression system for standard: E Coli, Immunogen sequence: I23-Y127) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL28 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL28 amount of sample captured in plate. Background: CCL28, also known as mucosae-associated epithelial chemokine (MEC), CCK1 and SCYA28, is a chemokine. It is mapped to 5p12. The tumor hypoxia promotes the recruitment of regulatory T (Treg) cells through induction of expression of the chemokine CCL28, which in turn promotes tumor tolerance and angiogenesis. CCL28 regulates the chemotaxis of cells that express the chemokine receptors CCR3 and CCR10. CCL28 has also been implicated in the migration of IgA-expressing cells to the mammary gland, salivary gland, intestine and other mucosal tissues. It has also been shown as a potential antimicrobial agent effective against certain pathogens, such as Gram negative and Gram positive bacteria and the fungus Candida albicans.
Human C-C motif chemokine 28 Protein information
Uniprot ID CCL28_HUMAN
Uniprot AC Q9NRJ3; D7RIE7;
UniGene Hs.656904;
GeneID 56477
KEGG hsa:56477;
Human C-C motif chemokine 28 Protein SEQUENCE
SEQUENCE 127 AA; 14280 MW; 3E8551A63A2C8D62 CRC64;
MQQRGLAIVA LAVCAALHAS EAILPIASSC CTEVSHHISR RLLERVNMCR
IQRADGDCDL AAVILHVKRR RICVSPHNHT VKQWMKVQAA KKNGKGNVCH
RKKHHGKRNS NRAHQGKHET YGHKTPY
UCSC uc003jnu.4; human. [Q9NRJ3-1];

Human CCL28 ELISA Kit

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