Human CCL22 ELISA Kit

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  • Alternative name

    Human CC chemokine STCP-1 ELISA Kit;Human MDC(1-69) ELISA Kit;Human macrophage-derived chemokine ELISA Kit;Human Small-inducible cytokine A22 ELISA Kit;Human Stimulated T-cell chemotactic protein 1 ELISA Kit;Human MDC ELISA Kit;Human SCYA22 ELISA Kit;Human A-152E5.1 ELISA Kit;Human ABCD-1 ELISA Kit;Human DC/B-CK ELISA Kit;Human STCP-1 ELISA Kit;Human C-C motif chemokine ligand 22 ELISA Kit;Human C-C motif chemokine 22 ELISA Kit;Human chemokine (C-C motif) ligand 22 ELISA Kit;Human small inducible cytokine A22 ELISA Kit;Human small inducible cytokine subfamily A (Cys-Cys), member 22 ELISA Kit;Human stimulated T cell chemotactic protein 1 ELISA Kit;

  • Catalog
    E007206
  • species
    Human
  • GeneCCL22
  • Standard CurveHuman CCL22 ELISA Kit
  • Other Species Human MDC/CCL22 ELISA KitHuman MDC / CCL22 ELISA KitMouse Ccl22 ELISA KitMouse MDC / CCL22 ELISA KitMouse MDC/CCL22 ELISA Kit
  • SpecificityNatural and recombinant human MDC
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 1pg/ml
  • Intended UseHuman CCL22 ELISA Kit allows for the in vitro quantitative determination of CCL22 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: human MDC ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MDC has been precoated onto 96-well plates. Standards(E.coli, G25-Q93) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MDC is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MDC amount of sample captured in plate. Background: Macrophage-derived chemokine (MDC), also called Chemokine, cc motif, ligand 22 (CCL22) or Small inducible cytokine subfamily A, member 22 (SCY22). MDC is a recently identified member of the CC chemokine family. It is not closely related to other chemokines, sharing most similarity with thymus- and activation-regulated chemokine (TARC), which contains 37% identical amino acids. In addition, MDC gene is mapped to chromosome 16q13, the same position reported for the TARC gene. MDC has the four-cysteine motif and other highly conserved residues characteristic of CC chemokines, but it shares <35% identity with any of the known chemokines. Recombinant MDC was expressed in Chinese hamster ovary cells and purified by heparin-Sepharose chromatography. MDC is highly expressed in macrophages and in monocyte-derived dendritic cells, but not in monocytes, natural killer cells, or several cell lines of epithelial, endothelial, or fibroblast origin. High expression was also detected in normal thymus and less expression in lung and spleen. MDC is thus a unique member of the CC chemokine family that may play a fundamental role in the function of dendritic cells, natural killer cells, and monocytes.
  • Human MDC(7-69) Protein information
  • Uniprot ID CCL22_HUMAN
  • Uniprot AC O00626; A0N0Q6; B2R4W2;
  • UniGene Hs.534347;
  • GeneID 6367
  • KEGG hsa:6367;
  • Human MDC(7-69) Protein SEQUENCE
  • SEQUENCE 93 AA; 10625 MW; 641FCE999586A7D2 CRC64;

    MDRLQTALLV VLVLLAVALQ ATEAGPYGAN MEDSVCCRDY VRYRLPLRVV

    KHFYWTSDSC PRPGVVLLTF RDKEICADPR VPWVKMILNK LSQ

  • UCSC uc002elh.4; human.;



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