Human CCL21 ELISA Kit

Alternative name
Human 6Ckine ELISA Kit;Human Beta-chemokine exodus-2 ELISA Kit;Human Secondary lymphoid-tissue chemokine ELISA Kit;Human SLC ELISA Kit;Human Small-inducible cytokine A21 ELISA Kit;Human SCYA21 ELISA Kit;Human CKb9 ELISA Kit;Human ECL ELISA Kit;Human TCA4 ELISA Kit;Human C-C motif chemokine ligand 21 ELISA Kit;Human C-C motif chemokine 21 ELISA Kit;Human Efficient Chemoattractant for Lymphocytes ELISA Kit;Human beta chemokine exodus-2 ELISA Kit;Human chemokine (C-C motif) ligand 21 ELISA Kit;Human secondary lymphoid tissue chemokine ELISA Kit;Human small inducible cytokine subfamily A (Cys-Cys), member 21 ELISA Kit;
Catalog
E007204
species
Human
GeneCCL21
SpecificityNatural and recombinant human CCL21
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 12pg/ml
Intended UseHuman CCL21 ELISA Kit allows for the in vitro quantitative determination of CCL21 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human CCL21 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL21 has been precoated onto 96-well plates. Standards(E.coli, S24-P134) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL21 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL21 amount of sample captured in plate. Background: Chemokine (C-C motif) ligand 21 (CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine (because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine (SLC).1 By somatic cell hybrid andradiation hybrid analyses, mapped the SCYA21 gene to 9p13. 2 Chemokines are a family of proteins that directleukocyte migration and activation to inflammatory stimuli. CXC chemokine ligand 13 (CXCL13), CC chemokine ligand 21 (CCL21), and CCL19 are constitutively expressed in secondary lymphoid organs, where they control the placement of lymphocytes and dendritic cells. It was demonstrate that the local expression of homeostatic chemokines in nonlymphoid organs, such as the lung, plays an important role in protective immune responses.3
Human C-C motif chemokine 21 Protein information
Uniprot ID CCL21_HUMAN
Uniprot AC O00585;
UniGene Hs.57907;
GeneID 6366
KEGG hsa:6366;
Human C-C motif chemokine 21 Protein SEQUENCE
SEQUENCE 134 AA; 14646 MW; FB48C2F71CB4A9D2 CRC64;
MAQSLALSLL ILVLAFGIPR TQGSDGGAQD CCLKYSQRKI PAKVVRSYRK
QEPSLGCSIP AILFLPRKRS QAELCADPKE LWVQQLMQHL DKTPSPQKPA
QGCRKDRGAS KTGKKGKGSK GCKRTERSQT PKGP

Human CCL21 ELISA Kit

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