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Catalog
E071653
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species
Mouse
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GeneTWEAK
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Standard Curve
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Other Species
Human TWEAK ELISA Kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Tweak. No significant cross-reactivity or interference between Tweak and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Tweak and all the analogues, therefore, cross reaction may still exist in some cases.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity1.0 pg/mL.
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Intended UseMouse TWEAK ELISA Kit allows for the in vitro quantitative determination of TWEAK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyCancer
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Product Description
specificalPrinciple of the assay: Tweak ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for Tweak. Standards or samples are then added to the microtiter plate wells and Tweak if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of Tweak present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for Tweak are added to each well to "sandwich" the Tweak immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Tweak and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Tweak concentration in each sample is interpolated from this standard curve.
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