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Catalog
E031105
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species
Human
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GenePro
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Pro. No significant cross-reactivity or interference between Pro and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Pro and all the analogues, therefore, cross reaction may still exist in some cases.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.1 ng/mL.
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Intended UseHuman Pro ELISA Kit allows for the in vitro quantitative determination of Pro , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalIntended Uses: This Pro ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human Pro. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay: Pro ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Pro antibody and an Pro-HRP conjugate. The assay sample and buffer are incubated together with Pro-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Pro concentration since Pro from samples and Pro-HRP conjugate compete for the anti-Pro antibody binding site. Since the number of sites is limited, as more sites are occupied by Pro from the sample, fewer sites are left to bind Pro-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Pro concentration in each sample is interpolated from this standard curve.
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