Human SESN2 ELISA Kit

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  • Alternative name

    Human Hypoxia-induced gene ELISA Kit;Human HI95 ELISA Kit;Human SES2 ELISA Kit;Human SEST2 ELISA Kit;Human sestrin 2 ELISA Kit;Human sestrin-2 ELISA Kit;Human hypoxia induced gene 95 ELISA Kit;Human hypoxia-induced ELISA Kit;

  • Catalog
    E034975
  • species
    Human
  • GeneSESN2
  • Standard CurveHuman SESN2 ELISA Kit
  • Other Species Mouse Sesn2 ELISA KitBovine SESN2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Sestrin 2 (SESN2). No significant cross-reactivity or interference between Sestrin 2 (SESN2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.054ng/mL.
  • Intended UseHuman SESN2 ELISA Kit allows for the in vitro quantitative determination of SESN2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sestrin 2 (SESN2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sestrin 2 (SESN2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sestrin 2 (SESN2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sestrin 2 (SESN2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Sestrin-2 Protein information
  • Uniprot ID SESN2_HUMAN
  • Uniprot AC P58004; Q5T7D0; Q96SI5;
  • UniGene Hs.469543;
  • GeneID 83667
  • KEGG hsa:83667;
  • Human Sestrin-2 Protein SEQUENCE
  • SEQUENCE 480 AA; 54494 MW; 9C13371316D84060 CRC64;

    MIVADSECRA ELKDYLRFAP GGVGDSGPGE EQRESRARRG PRGPSAFIPV

    EEVLREGAES LEQHLGLEAL MSSGRVDNLA VVMGLHPDYF TSFWRLHYLL

    LHTDGPLASS WRHYIAIMAA ARHQCSYLVG SHMAEFLQTG GDPEWLLGLH

    RAPEKLRKLS EINKLLAHRP WLITKEHIQA LLKTGEHTWS LAELIQALVL

    LTHCHSLSSF VFGCGILPEG DADGSPAPQA PTPPSEQSSP PSRDPLNNSG

    GFESARDVEA LMERMQQLQE SLLRDEGTSQ EEMESRFELE KSESLLVTPS

    ADILEPSPHP DMLCFVEDPT FGYEDFTRRG AQAPPTFRAQ DYTWEDHGYS

    LIQRLYPEGG QLLDEKFQAA YSLTYNTIAM HSGVDTSVLR RAIWNYIHCV

    FGIRYDDYDY GEVNQLLERN LKVYIKTVAC YPEKTTRRMY NLFWRHFRHS

    EKVHVNLLLL EARMQAALLY ALRAITRYMT

  • UCSC uc001bps.4; human.;


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