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Alternative name
C-X-C chemokine receptor type 1 ELISA KIT; CDw128a ELISA KIT; High affinity interleukin-8 receptor A ELISA KIT; IL-8R A ELISA KIT; IL-8 receptor type 1 ELISA KIT; CD_antigen: CD181CXCR1 ELISA KIT; CMKAR1 ELISA KIT; IL8RA ELISA KIT; CXC-R1 ELISA KIT; CXCR-1 ELISA KIT; IL-8R A ELISA KIT
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Catalog
E020796
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species
Human
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GeneIL8Ra
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Interleukin 8 Receptor Alpha (IL8Ra).
No significant cross-reactivity or interference between Interleukin 8 Receptor Alpha (IL8Ra) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 0.058ng/mL.
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Intended UseHuman IL8Ra ELISA Kit allows for the in vitro quantitative determination of IL8Ra , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 8 Receptor Alpha (IL8Ra). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 8 Receptor Alpha (IL8Ra). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 8 Receptor Alpha (IL8Ra), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 8 Receptor Alpha (IL8Ra) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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