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Alternative name
Thrombopoietin receptor ELISA KIT; Myeloproliferative leukemia protein ELISA KIT; Proto-oncogene c-Mpl ELISA KIT; CD_antigen: CD110MPL ELISA KIT; TPOR ELISA KIT; TPO-R ELISA KIT
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Catalog
E070019
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species
Mouse
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GeneTPORAb
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.1ng/ml
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Intended UseMouse TPORAb ELISA Kit allows for the in vitro quantitative determination of TPORAb , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyImmunology
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Product Description
specificalFor samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
INTENDED USE This Anti-TPOR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse Anti-TPOR. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
PRINCIPLE OF THE ASSAY Anti-TPOR ELISA kit applies the competitive enzyme immunoassay technique utilizing Thrombopoietin Receptor antigen and an Anti-TPOR-HRP conjugate. The assay sample and buffer are incubated together with Anti-TPOR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Anti-TPOR concentration since Anti-TPOR from samples and Anti-TPOR-HRP conjugate compete for the Thrombopoietin Receptor antigen binding site. Since the number of sites is limited, as more sites are occupied by Anti-TPOR from the sample, fewer sites are left to bind Anti-TPOR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-TPOR concentration in each sample is interpolated from this standard curve.
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