Human ATP2A1 ELISA Kit

Alternative name
Human SERCA1 ELISA Kit;Human SR Ca(2+)-ATPase 1 ELISA Kit;Human calcium pump 1 ELISA Kit;Human calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform ELISA Kit;Human endoplasmic reticulum class 1/2 Ca(2+) ATPase ELISA Kit;Human ATP2A ELISA Kit;Human ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1 ELISA Kit;Human sarcoplasmic/endoplasmic reticulum calcium ATPase 1 ELISA Kit;Human ATPase, Ca++ transporting, cardiac muscle, fast twitch 1 ELISA Kit;
Catalog
E004152
species
Human
GeneATP2A1
Standard Curve
Other Species Mouse Atp2a1 ELISA Kit Bovine ATP2A1 ELISA Kit Chicken ATP2A1 ELISA Kit Rabbit ATP2A1 ELISA Kit Rat Atp2a1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of human ATP2A1. No significant cross-reactivity or interference between human ATP2A1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human ATP2A1 is typically less than 4.69 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
Intended UseHuman ATP2A1 ELISA Kit allows for the in vitro quantitative determination of ATP2A1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ATP2A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ATP2A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ATP2A1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ATP2A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 Protein information
Uniprot ID AT2A1_HUMAN
Uniprot AC O14983; A8K5J9; B3KY17; O14984;
UniGene Hs.657344;
GeneID 487
KEGG hsa:487;
Human Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 Protein SEQUENCE
SEQUENCE 1001 AA; 110252 MW; C8F33809B56FDDEE CRC64;
MEAAHAKTTE ECLAYFGVSE TTGLTPDQVK RNLEKYGLNE LPAEEGKTLW
ELVIEQFEDL LVRILLLAAC ISFVLAWFEE GEETITAFVE PFVILLILIA
NAIVGVWQER NAENAIEALK EYEPEMGKVY RADRKSVQRI KARDIVPGDI
VEVAVGDKVP ADIRILAIKS TTLRVDQSIL TGESVSVIKH TEPVPDPRAV
NQDKKNMLFS GTNIAAGKAL GIVATTGVGT EIGKIRDQMA ATEQDKTPLQ
QKLDEFGEQL SKVISLICVA VWLINIGHFN DPVHGGSWFR GAIYYFKIAV
ALAVAAIPEG LPAVITTCLA LGTRRMAKKN AIVRSLPSVE TLGCTSVICS
DKTGTLTTNQ MSVCKMFIID KVDGDICLLN EFSITGSTYA PEGEVLKNDK
PVRPGQYDGL VELATICALC NDSSLDFNEA KGVYEKVGEA TETALTTLVE
KMNVFNTDVR SLSKVERANA CNSVIRQLMK KEFTLEFSRD RKSMSVYCSP
AKSSRAAVGN KMFVKGAPEG VIDRCNYVRV GTTRVPLTGP VKEKIMAVIK
EWGTGRDTLR CLALATRDTP PKREEMVLDD SARFLEYETD LTFVGVVGML
DPPRKEVTGS IQLCRDAGIR VIMITGDNKG TAIAICRRIG IFGENEEVAD
RAYTGREFDD LPLAEQREAC RRACCFARVE PSHKSKIVEY LQSYDEITAM
TGDGVNDAPA LKKAEIGIAM GSGTAVAKTA SEMVLADDNF STIVAAVEEG
RAIYNNMKQF IRYLISSNVG EVVCIFLTAA LGLPEALIPV QLLWVNLVTD
GLPATALGFN PPDLDIMDRP PRSPKEPLIS GWLFFRYMAI GGYVGAATVG
AAAWWFLYAE DGPHVNYSQL THFMQCTEDN THFEGIDCEV FEAPEPMTMA
LSVLVTIEMC NALNSLSENQ SLLRMPPWVN IWLLGSICLS MSLHFLILYV
DPLPMIFKLR ALDLTQWLMV LKISLPVIGL DEILKFVARN YLEDPEDERR
K
UCSC uc002drn.1; human. [O14983-1];

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