SpecificityThis assay has high sensitivity and excellent specificity for detection of PTH. No significant cross-reactivity or interference between PTH and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PTH and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseHuman PTH ELISA Kit allows for the in vitro quantitative determination of PTH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyHuman ELISA Kit
Product Description specificalFor samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
INTENDED USE This PTH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PTH. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
PRINCIPLE OF THE ASSAY PTH ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PTH antibody and an PTH-HRP conjugate. The assay sample and buffer are incubated together with PTH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PTH concentration since PTH from samples and PTH-HRP conjugate compete for the anti-PTH antibody binding site. Since the number of sites is limited, as more sites are occupied by PTH from the sample, fewer sites are left to bind PTH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PTH concentration in each sample is interpolated from this standard curve.