Human CRTAC1 ELISA Kit

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  • Alternative name

    Human 68 kDa chondrocyte-expressed protein ELISA Kit;Human CEP-68 ELISA Kit;Human ASPIC ELISA Kit;Human ASPIC1 ELISA Kit;Human CEP68 ELISA Kit;Human cartilage acidic protein 1 ELISA Kit;Human acidic secreted protein in cartilage ELISA Kit;Human chondrocyte expressed protein 68 kDa CEP-68 ELISA Kit;

  • Catalog
    E006761
  • species
    Human
  • GeneCRTAC1
  • Standard CurveHuman CRTAC1 ELISA Kit
  • Other Species Mouse Crtac1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CRTAC1. No significant cross-reactivity or interference between CRTAC1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CRTAC1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman CRTAC1 ELISA Kit allows for the in vitro quantitative determination of CRTAC1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the assay: CRTAC1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CRTAC1 antibody and an CRTAC1-HRP conjugate. The assay sample and buffer are incubated together with CRTAC1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CRTAC1 concentration since CRTAC1 from samples and CRTAC1-HRP conjugate compete for the anti-CRTAC1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CRTAC1 from the sample, fewer sites are left to bind CRTAC1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CRTAC1 concentration in each sample is interpolated from this standard curve.
  • Human Cartilage acidic protein 1 Protein information
  • Uniprot ID CRAC1_HUMAN
  • Uniprot AC VDRKGSGR YSIYIANYAY GNVGPDALIE MDPEASDLSR GILALRDVAA EAGVSKYTGG
  • UniGene Hs.500736;
  • GeneID 55118
  • KEGG hsa:55118;
  • Human Cartilage acidic protein 1 Protein SEQUENCE
  • SEQUENCE 661 AA; 71421 MW; 2EF75321817F0D01 CRC64;

    MAPSADPGMS RMLPFLLLLW FLPITEGSQR AEPMFTAVTN SVLPPDYDSN

    PTQLNYGVAV TDVDHDGDFE IVVAGYNGPN LVLKYDRAQK RLVNIAVDER

    SSPYYALRDR QGNAIGVTAC DIDGDGREEI YFLNTNNAFS GVATYTDKLF

    KFRNNRWEDI LSDEVNVARG VASLFAGRSV ACVDRKGSGR YSIYIANYAY

    GNVGPDALIE MDPEASDLSR GILALRDVAA EAGVSKYTGG RGVSVGPILS

    SSASDIFCDN ENGPNFLFHN RGDGTFVDAA ASAGVDDPHQ HGRGVALADF

    NRDGKVDIVY GNWNGPHRLY LQMSTHGKVR FRDIASPKFS MPSPVRTVIT

    ADFDNDQELE IFFNNIAYRS SSANRLFRVI RREHGDPLIE ELNPGDALEP

    EGRGTGGVVT DFDGDGMLDL ILSHGESMAQ PLSVFRGNQG FNNNWLRVVP

    RTRFGAFARG AKVVLYTKKS GAHLRIIDGG SGYLCEMEPV AHFGLGKDEA

    SSVEVTWPDG KMVSRNVASG EMNSVLEILY PRDEDTLQDP APLECGQGFS

    QQENGHCMDT NECIQFPFVC PRDKPVCVNT YGSYRCRTNK KCSRGYEPNE

    DGTACVGTLG QSPGPRPTTP TAAAATAAAA AAAGAATAAP VLVDGDLNLG

    SVVKESCEPS C

  • UCSC uc001kou.2; human. [Q9NQ79-1];


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