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Human CASP7 ELISA Kit

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  • Alternative name

    Human CASP-7 ELISA Kit;Human apoptotic protease MCH-3 ELISA Kit;Human CMH-1 ELISA Kit;Human ICE-like apoptotic protease 3 ELISA Kit;Human ICE-LAP3 ELISA Kit;Human MCH3 ELISA Kit;Human LICE2 ELISA Kit;Human caspase 7 ELISA Kit;Human caspase-7 ELISA Kit;Human caspase 7, apoptosis-related cysteine peptidase ELISA Kit;Human caspase 7, apoptosis-related cysteine protease ELISA Kit;
  • Catalog
    E006881
  • species
    Human
  • GeneCASP7
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CASP7 ELISA Kit allows for the in vitro quantitative determination of CASP7 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical    Principle of the Assay: CASP7 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CASP7. Standards or samples are then added to the microtiter plate wells and CASP7 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CASP7 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for CASP7 are added to each well to "sandwich" the CASP7 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CASP7 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CASP7 concentration in each sample is interpolated from this standard curve.
  • Human Caspase-7 subunit p11 Protein information
  • Uniprot ID CASP7_HUMAN
  • Uniprot AC Q96BA0;
  • UniGene Hs.9216;
  • GeneID 840
  • KEGG hsa:840;
  • Human Caspase-7 subunit p11 Protein SEQUENCE

  • SEQUENCE 303 AA; 34277 MW; CD373EE54A232CA4 CRC64;

    MADDQGCIEE QGVEDSANED SVDAKPDRSS FVPSLFSKKK KNVTMRSIKT

    TRDRVPTYQY NMNFEKLGKC IIINNKNFDK VTGMGVRNGT DKDAEALFKC

    FRSLGFDVIV YNDCSCAKMQ DLLKKASEED HTNAACFACI LLSHGEENVI

    YGKDGVTPIK DLTAHFRGDR CKTLLEKPKL FFIQACRGTE LDDGIQADSG

    PINDTDANPR YKIPVEADFL FAYSTVPGYY SWRSPGRGSW FVQALCSILE

    EHGKDLEIMQ ILTRVNDRVA RHFESQSDDP HFHEKKQIPC VVSMLTKELY

    FSQ

  • UCSC uc001lam.5; human. [P55210-1];



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