Human NOS3/eNOS ELISA Kit

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  • Alternative name

    Nitric oxide synthase, endothelial ELISA KIT; Constitutive NOS ELISA KIT; cNOS ELISA KIT; EC-NOS ELISA KIT; Endothelial NOS ELISA KIT; eNOS ELISA KIT; NOS type III ELISA KIT; NOSIIINOS3 ELISA KIT; cNOS ELISA KIT; eNOS ELISA KIT; NOSIII ELISA KIT

  • Catalog
    E026790
  • species
    Human
  • GeneNOS3/eNOS
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human NOS3/eNOS. No significant cross-reactivity or interference between Human NOS3/eNOS and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human NOS3/eNOS and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity37.5pg/mL
  • Intended UseHuman NOS3/eNOS ELISA Kit allows for the in vitro quantitative determination of NOS3/eNOS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NOS3/eNOS in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NOS3/eNOS. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human NOS3/eNOS and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NOS3/eNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human NOS3/eNOS. You can calculate the concentration of Human NOS3/eNOS in the samples by comparing the OD of the samples to the standard curve.


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