Human GLUD1 ELISA Kit

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  • Alternative name

    Human GDH 1 ELISA Kit;Human GLUD ELISA Kit;Human GDH ELISA Kit;Human GDH1 ELISA Kit;Human glutamate dehydrogenase 1 ELISA Kit;Human glutamate dehydrogenase 1, mitochondrial ELISA Kit;Human epididymis tissue sperm binding protein Li 18mP ELISA Kit;Human glutamate dehydrogenase (NAD(P)+) ELISA Kit;

  • Catalog
    E016721
  • species
    Human
  • GeneGLUD1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human GLUD1. No significant cross-reactivity or interference between human GLUD1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human GLUD1 is typically less than 2.34 uU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
  • Intended UseHuman GLUD1 ELISA Kit allows for the in vitro quantitative determination of GLUD1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GLUD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GLUD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GLUD1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GLUD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Glutamate dehydrogenase 1, mitochondrial Protein information
  • Uniprot ID DHE3_HUMAN
  • Uniprot AC P00367; B3KV55; B4DGN5; Q5TBU3;
  • UniGene Hs.500409; Hs.731740;
  • GeneID 2746
  • KEGG hsa:2746;
  • Human Glutamate dehydrogenase 1, mitochondrial Protein SEQUENCE
  • SEQUENCE 558 AA; 61398 MW; A7319A840F57FBB2 CRC64;

    MYRYLGEALL LSRAGPAALG SASADSAALL GWARGQPAAA PQPGLALAAR

    RHYSEAVADR EDDPNFFKMV EGFFDRGASI VEDKLVEDLR TRESEEQKRN

    RVRGILRIIK PCNHVLSLSF PIRRDDGSWE VIEGYRAQHS QHRTPCKGGI

    RYSTDVSVDE VKALASLMTY KCAVVDVPFG GAKAGVKINP KNYTDNELEK

    ITRRFTMELA KKGFIGPGID VPAPDMSTGE REMSWIADTY ASTIGHYDIN

    AHACVTGKPI SQGGIHGRIS ATGRGVFHGI ENFINEASYM SILGMTPGFG

    DKTFVVQGFG NVGLHSMRYL HRFGAKCIAV GESDGSIWNP DGIDPKELED

    FKLQHGSILG FPKAKPYEGS ILEADCDILI PAASEKQLTK SNAPRVKAKI

    IAEGANGPTT PEADKIFLER NIMVIPDLYL NAGGVTVSYF EWLKNLNHVS

    YGRLTFKYER DSNYHLLMSV QESLERKFGK HGGTIPIVPT AEFQDRISGA

    SEKDIVHSGL AYTMERSARQ IMRTAMKYNL GLDLRTAAYV NAIEKVFKVY

    NEAGVTFT

  • UCSC uc001keh.4; human. [P00367-1];



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