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Alternative name
Human REPRIMO ELISA Kit;Human reprimo, TP53 dependent G2 arrest mediator homolog ELISA Kit;Human protein reprimo ELISA Kit;Human candidate mediator of the p53 dependent G2 arrest ELISA Kit;Human reprimo, TP53 dependant G2 arrest mediator candidate ELISA Kit;Human reprimo, TP53 dependent G2 arrest mediator candidate ELISA Kit;
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Catalog
E031771
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species
Human
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GeneRPRM
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity<0.094ng/ml
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Intended UseHuman RPRM ELISA Kit allows for the in vitro quantitative determination of RPRM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human RPRMantibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human RPRM antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human RPRM amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman RPRMcan be calculated.
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Human Protein reprimo Protein information
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Uniprot ID
RPRM_HUMAN
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Uniprot AC
Q9NS64; B2R4V1;
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UniGene
Hs.100890;
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GeneID
56475
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KEGG
hsa:56475;
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Human Protein reprimo Protein SEQUENCE
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SEQUENCE 109 AA; 11774 MW; 7026A0AB1FB6BE62 CRC64;
MNPALGNQTD VAGLFLANSS EALERAVRCC TQASVVTDDG FAEGGPDERS
LYIMRVVQIA
VMCVLSLTVV FGIFFLGCNL LIKSEGMINF LVKDRRPSKE
VEAVVVGPY
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UCSC
uc002tyq.1; human.;
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