Human CR ELISA Kit

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  • Alternative name

    Calretinin ELISA KIT; 29 kDa calbindinCALB2 ELISA KIT; CAB29 ELISA KIT; CR ELISA KIT

  • Catalog
    E006250
  • species
    Human
  • GeneCR
  • Standard CurveHuman CR ELISA Kit
  • Other Species Human Cr ELISA KitMouse Cr ELISA KitBovine Cr ELISA KitCanine Cr ELISA KitChicken Cr ELISA KitGeneral Cr ELISA KitPorcine Cr ELISA KitRabbit Cr ELISA KitRat Cr ELISA KitCamel Cr ELISA KitGoat Cr ELISA KitGuinea Pig Cr ELISA KitHamster Cr ELISA KitHorse Cr ELISA KitMonkey Cr ELISA KitSheep Cr ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman CR ELISA Kit allows for the in vitro quantitative determination of CR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyNeurobiology
  • Product Description
    specifical
    Principle of the Assay: CR ELISA kit applies the quantitative sandwich enzyme imniunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CR. Standards or samples are then added to the microtiter plate wells and CR if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CR present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody specific for CR are added to each well to "sandwich" the CR immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CR and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotonietrically at a wavelength of 450 mn. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CR concentration in each sample is interpolated from this standard curve.




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