-
Alternative name
Nephrin ELISA KIT; Renal glomerulus-specific cell adhesion receptorNPHS1 ELISA KIT; NPHN ELISA KIT
-
Catalog
E061226
-
species
Mouse
-
GeneNephrin
-
Standard Curve
-
Other Species
Human Nephrin ELISA Kit
-
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
-
Sensitivity0.1 ng/mL.
-
Intended UseMouse Nephrin ELISA Kit allows for the in vitro quantitative determination of Nephrin , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
-
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
-
Product Description
specificalPrinciple of the Assay: NPHN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NPHN antibody and an NPHN-HRP conjugate. The assay sample and buffer are incubated together with NPHN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NPHN concentration since NPHN from samples and NPHN-HRP conjugate compete for the anti-NPHN antibody binding site. Since the number of sites is limited, as more sites are occupied by NPHN from the sample, fewer sites are left to bind NPHN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NPHN concentration in each sample is interpolated from this standard curve.
-