Human CDK5 ELISA Kit

Alternative name
Human cell division protein kinase 5 ELISA Kit;Human serine/threonine-protein kinase PSSALRE ELISA Kit;Human tau protein kinase II catalytic subunit ELISA Kit;Human TPKII catalytic subunit ELISA Kit;Human CDKN5 ELISA Kit;Human LIS7 ELISA Kit;Human PSSALRE ELISA Kit;Human cyclin dependent kinase 5 ELISA Kit;Human cyclin-dependent-like kinase 5 ELISA Kit;Human protein kinase CDK5 splicing ELISA Kit;
Catalog
E010572
species
Human
GeneCDK5
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CDK5 ELISA Kit allows for the in vitro quantitative determination of CDK5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalPrinciple of the Assay: CDK5 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CDK5. Standards or samples are then added to the microtiter plate wells and CDK5 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CDK5 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for CDK5 are added to each well to "sandwich" the CDK5 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CDK5 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDK5 concentration in each sample is interpolated from this standard curve.
Human Cyclin-dependent-like kinase 5 Protein information
Uniprot ID CDK5_HUMAN
Uniprot AC Q00535; A1XKG3;
UniGene Hs.647078;
GeneID 1020
KEGG hsa:1020;
Human Cyclin-dependent-like kinase 5 Protein SEQUENCE
SEQUENCE 292 AA; 33304 MW; 54D10495F017D527 CRC64;
MQKYEKLEKI GEGTYGTVFK AKNRETHEIV ALKRVRLDDD DEGVPSSALR
EICLLKELKH KNIVRLHDVL HSDKKLTLVF EFCDQDLKKY FDSCNGDLDP
EIVKSFLFQL LKGLGFCHSR NVLHRDLKPQ NLLINRNGEL KLADFGLARA
FGIPVRCYSA EVVTLWYRPP DVLFGAKLYS TSIDMWSAGC IFAELANAGR
PLFPGNDVDD QLKRIFRLLG TPTEEQWPSM TKLPDYKPYP MYPATTSLVN
VVPKLNATGR DLLQNLLKCN PVQRISAEEA LQHPYFSDFC PP
UCSC uc003wir.3; human. [Q00535-1];

Human CDK5 ELISA Kit

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