Human FNDC5 ELISA Kit

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  • Alternative name

    Human fibronectin type III repeat-containing protein 2 ELISA Kit;Human FRCP2 ELISA Kit;Human irisin ELISA Kit;Human fibronectin type III domain containing 5 ELISA Kit;Human fibronectin type III domain-containing protein 5 ELISA Kit;

  • Catalog
    E015067
  • species
    Human
  • GeneFNDC5
  • Other Species Mouse Fndc5 ELISA KitMouse FNDC5/FRCP2 ELISA KitRat Fndc5 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human FNDC5. No significant cross-reactivity or interference between human FNDC5 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human FNDC5 is typically less than 1.95 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
  • Intended UseHuman FNDC5 ELISA Kit allows for the in vitro quantitative determination of FNDC5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FNDC5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FNDC5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FNDC5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FNDC5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Irisin Protein information
  • Uniprot ID FNDC5_HUMAN
  • Uniprot AC Q8NAU1; A6NMC9; D3DPQ6; Q6P6D9; Q7Z676;
  • UniGene Hs.524234;
  • GeneID 252995
  • KEGG hsa:252995;
  • Human Irisin Protein SEQUENCE
  • SEQUENCE 212 AA; 23659 MW; C4A066452A05A134 CRC64;

    MHPGSPSAWP PRARAALRLW LGCVCFALVQ ADSPSAPVNV TVRHLKANSA

    VVSWDVLEDE VVIGFAISQQ KKDVRMLRFI QEVNTTTRSC ALWDLEEDTE

    YIVHVQAISI QGQSPASEPV LFKTPREAEK MASKNKDEVT MKEMGRNQQL

    RTGEVLIIVV VLFMWAGVIA LFCRQYDIIK DNEPNNNKEK TKSASETSTP

    EHQGGGLLRS KI

  • UCSC uc001bwg.5; human. [Q8NAU1-1];


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