Human FNDC5 ELISA Kit

Alternative name
Human fibronectin type III repeat-containing protein 2 ELISA Kit;Human FRCP2 ELISA Kit;Human irisin ELISA Kit;Human fibronectin type III domain containing 5 ELISA Kit;Human fibronectin type III domain-containing protein 5 ELISA Kit;
Catalog
E015067
species
Human
GeneFNDC5
SpecificityThis assay has high sensitivity and excellent specificity for detection of human FNDC5. No significant cross-reactivity or interference between human FNDC5 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human FNDC5 is typically less than 1.95 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
Intended UseHuman FNDC5 ELISA Kit allows for the in vitro quantitative determination of FNDC5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FNDC5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FNDC5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FNDC5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FNDC5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Irisin Protein information
Uniprot ID FNDC5_HUMAN
Uniprot AC Q8NAU1; A6NMC9; D3DPQ6; Q6P6D9; Q7Z676;
UniGene Hs.524234;
GeneID 252995
KEGG hsa:252995;
Human Irisin Protein SEQUENCE
SEQUENCE 212 AA; 23659 MW; C4A066452A05A134 CRC64;
MHPGSPSAWP PRARAALRLW LGCVCFALVQ ADSPSAPVNV TVRHLKANSA
VVSWDVLEDE VVIGFAISQQ KKDVRMLRFI QEVNTTTRSC ALWDLEEDTE
YIVHVQAISI QGQSPASEPV LFKTPREAEK MASKNKDEVT MKEMGRNQQL
RTGEVLIIVV VLFMWAGVIA LFCRQYDIIK DNEPNNNKEK TKSASETSTP
EHQGGGLLRS KI
UCSC uc001bwg.5; human. [Q8NAU1-1];

Category

Human FNDC5 ELISA Kit

Human fibronectin type III repeat-containing protein 2 ELISA Kit;Human FRCP2 ELISA Kit;Human irisin ELISA Kit;Human fibronectin type III domain containing 5 ELISA Kit;Human fibronectin type III domain-containing protein 5 ELISA Kit;