Human DRD4 ELISA Kit

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  • Alternative name

    Human D(2C) dopamine receptor ELISA Kit;Human dopamine D4 receptor ELISA Kit;Human D4DR ELISA Kit;Human dopamine receptor D4 ELISA Kit;Human D(4) dopamine receptor ELISA Kit;Human seven transmembrane helix receptor ELISA Kit;

  • Catalog
    E012318
  • species
    Human
  • GeneDRD4
  • Other Species Human D4R/DRD4 ELISA KitMouse Drd4 ELISA KitRat Drd4 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman DRD4 ELISA Kit allows for the in vitro quantitative determination of DRD4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyNeurobiology
  • Product Description
    specifical
    Principle of the Assay: DRD4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for DRD4. Standards or samples are then added to the microtiter plate wells and DRD4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of DRD4 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for DRD4 are added to each well to "sandwich" the DRD4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain DRD4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DRD4 concentration in each sample is interpolated from this standard curve.
  • Human D(4) dopamine receptor Protein information
  • Uniprot ID DRD4_HUMAN
  • Uniprot AC P21917; B0M0J7; Q7Z7Q5; Q8NGM5;
  • UniGene Hs.99922;
  • GeneID 1815
  • KEGG hsa:1815;
  • Human D(4) dopamine receptor Protein SEQUENCE
  • SEQUENCE 467 AA; 48361 MW; B6FF2E09269A02AF CRC64;

    MGNRSTADAD GLLAGRGPAA GASAGASAGL AGQGAAALVG GVLLIGAVLA

    GNSLVCVSVA TERALQTPTN SFIVSLAAAD LLLALLVLPL FVYSEVQGGA

    WLLSPRLCDA LMAMDVMLCT ASIFNLCAIS VDRFVAVAVP LRYNRQGGSR

    RQLLLIGATW LLSAAVAAPV LCGLNDVRGR DPAVCRLEDR DYVVYSSVCS

    FFLPCPLMLL LYWATFRGLQ RWEVARRAKL HGRAPRRPSG PGPPSPTPPA

    PRLPQDPCGP DCAPPAPGLP RGPCGPDCAP AAPGLPPDPC GPDCAPPAPG

    LPQDPCGPDC APPAPGLPRG PCGPDCAPPA PGLPQDPCGP DCAPPAPGLP

    PDPCGSNCAP PDAVRAAALP PQTPPQTRRR RRAKITGRER KAMRVLPVVV

    GAFLLCWTPF FVVHITQALC PACSVPPRLV SAVTWLGYVN SALNPVIYTV

    FNAEFRNVFR KALRACC

  • UCSC uc001lqp.3; human.;


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