SensitivityThe minimum detectable dose of mouse MAP1LC3B is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the
Intended UseMouse Map1lc3b ELISA Kit allows for the in vitro quantitative determination of Map1lc3b , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MAP1LC3B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAP1LC3B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAP1LC3B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAP1LC3B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Microtubule-associated proteins 1A/1B light chain 3B Protein information
Q9CQV6; Q3U9W5; Q9D1R0;
Mouse Microtubule-associated proteins 1A/1B light chain 3B Protein SEQUENCE