SpecificityEach crossreactant is spiked into a sample containing Calcitonin. Calcitonin level is measured before and after the spike. None of the crossreactants interfere with this Calcitonin ELISA. The small changes in Calcitonin measured are well within the intraassay precision statistics.
SensitivityThe sensitivity, or minimum detection limit, of this assay is defined as the smallest single value, which can be distinguished from zero at the 95% confidence limit. The Calcitonin ELISA has a calculated sensitivity of 1.0 pg/mL.
Intended UseHuman CALCA ELISA Kit allows for the in vitro quantitative determination of CALCA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyEndocrinology ; Hormones; Bone & Mineral Metabolism; Diagnostic Assays; Assay Categories
Product Description specificalPrinciple of the assay: The Calcitonin Immunoassay is a two-site ELISA [Enzyme-Linked ImmunoSorbent Assay] for the measurement of the biologically intact 32 amino acid chain of Calcitonin. It utilizes two different mouse monoclonal antibodies to human calcitonin specific for well-defined regions on the calcitonin molecule. One antibody binds only to Calcitonin 11-23 and this antibody is biotinylated. The other antibody binds only to Calcitonin 21-32 and this antibody is labeled with horseradish peroxidase [HRP] for detection. In this assay, calibrators, controls, or patient samples are simultaneously incubated with the enzyme labeled antibody and a biotin coupled antibody in a streptavidin-coated microplate well. Thus the calcitonin in the sample is "sandwiched" between these two antibodies. At the end of the assay incubation, the microwell is washed to remove unbound components and the enzyme bound to the solid phase is incubated with the substrate, tetramethylbenzidine (TMB). An acidic stopping solution is then added to stop the reaction and converts the color to yellow. The intensity of the yellow color is directly proportional to the concentration of calcitonin in the sample. A dose response curve of absorbance unit vs. concentration is generated using results obtained from the calibrators. Concentrations of calcitonin present in the controls and patient samples are determined directly from this curve.
Human Katacalcin Protein information
P01258; B7ZL39; Q13935; Q13937; Q52LX7;