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Alternative name
Human CC chemokine IMAC ELISA Kit;Human eotaxin-3 ELISA Kit;Human macrophage inflammatory protein 4-alpha ELISA Kit;Human MIP-4-alpha ELISA Kit;Human Small-inducible cytokine A26 ELISA Kit;Human thymic stroma chemokine-1 ELISA Kit;Human TSC-1 ELISA Kit;Human SCYA26 ELISA Kit;Human IMAC ELISA Kit;Human MIP-4a ELISA Kit;Human MIP-4alpha ELISA Kit;Human C-C motif chemokine ligand 26 ELISA Kit;Human C-C motif chemokine 26 ELISA Kit;Human chemokine (C-C motif) ligand 26 ELISA Kit;Human chemokine N1 ELISA Kit;Human small inducible cytokine A26 ELISA Kit;Human small inducible cytokine subfamily A (Cys-Cys), member 26 ELISA Kit;
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Catalog
E005449
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species
Human
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GeneCCL26
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Standard Curve
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Other Species
Mouse CCL26 ELISA KitCanine CCL26 ELISA Kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of CCL-26. No significant cross-reactivity or interference between CCL-26 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CCL-26 and all the analogues, therefore, cross reaction may still exist in some cases.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity1.0 pg/mL.
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Intended UseHuman CCL26 ELISA Kit allows for the in vitro quantitative determination of CCL26 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Categories/FamilyImmunology
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Product Description
specificalPrinciple of the assay: CCL-26 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CCL-26 antibody and an CCL-26-HRP conjugate. The assay sample and buffer are incubated together with CCL-26-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCL-26 concentration since CCL-26 from samples and CCL-26-HRP conjugate compete for the anti-CCL-26 antibody binding site. Since the number of sites is limited, as more sites are occupied by CCL-26 from the sample, fewer sites are left to bind CCL-26-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCL-26 concentration in each sample is interpolated from this standard curve.
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Human C-C motif chemokine 26 Protein information
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Uniprot ID
CCL26_HUMAN
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Uniprot AC
Q9Y258; A0N0Q5; Q52LV8;
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UniGene
Hs.131342;
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GeneID
10344
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KEGG
hsa:10344;
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Human C-C motif chemokine 26 Protein SEQUENCE
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SEQUENCE 94 AA; 10648 MW; 8525B1E4BDD39A5F CRC64;
MMGLSLASAV LLASLLSLHL GTATRGSDIS KTCCFQYSHK PLPWTWVRSY
EFTSNSCSQR
AVIFTTKRGK KVCTHPRKKW VQKYISLLKT PKQL
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UCSC
uc003udt.2; human.;
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