SpecificityThis assay has high sensitivity and excellent specificity for detection of CCL-26. No significant cross-reactivity or interference between CCL-26 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CCL-26 and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseHuman CCL26 ELISA Kit allows for the in vitro quantitative determination of CCL26 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the assay: CCL-26 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CCL-26 antibody and an CCL-26-HRP conjugate. The assay sample and buffer are incubated together with CCL-26-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCL-26 concentration since CCL-26 from samples and CCL-26-HRP conjugate compete for the anti-CCL-26 antibody binding site. Since the number of sites is limited, as more sites are occupied by CCL-26 from the sample, fewer sites are left to bind CCL-26-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCL-26 concentration in each sample is interpolated from this standard curve.