Mouse Fgf21 ELISA Kit

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  • Alternative name

    Mouse FGF-21 ELISA Kit;Mouse fibroblast growth factor 21 ELISA Kit;

  • Catalog
    E052895
  • species
    Mouse
  • GeneFgf21
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse FGF21. No significant cross-reactivity or interference between Mouse FGF21 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse FGF21 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity18.75pg/mL
  • Intended UseMouse Fgf21 ELISA Kit allows for the in vitro quantitative determination of Fgf21 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FGF21 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse FGF21. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse FGF21 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FGF21, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse FGF21. You can calculate the concentration of Mouse FGF21 in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Fibroblast growth factor 21 Protein information
  • Uniprot ID FGF21_MOUSE
  • Uniprot AC SFRELLLE DGYNVYQSEA HGLPLRLPQK DSPNQDATSW GPVRFLPMPG LLHEPQDQAG
  • UniGene Mm.143736;
  • GeneID 56636
  • KEGG mmu:56636;
  • Mouse Fibroblast growth factor 21 Protein SEQUENCE
  • SEQUENCE 210 AA; 23237 MW; AE02AABA6477E6F0 CRC64;

    MEWMRSRVGT LGLWVRLLLA VFLLGVYQAY PIPDSSPLLQ FGGQVRQRYL

    YTDDDQDTEA HLEIREDGTV VGAAHRSPES LLELKALKPG VIQILGVKAS

    RFLCQQPDGA LYGSPHFDPE ACSFRELLLE DGYNVYQSEA HGLPLRLPQK

    DSPNQDATSW GPVRFLPMPG LLHEPQDQAG FLPPEPPDVG SSDPLSMVEP

    LQGRSPSYAS

  • UCSC uc009gwe.1; mouse.;



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