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  • Catalog
  • species
  • GeneMMSAM
  • Standard CurveHuman MMSAM ELISA Kit
  • Other Species Mouse MMSAM ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman MMSAM ELISA Kit allows for the in vitro quantitative determination of MMSAM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyCell Biology
  • Product Description
    For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate INTENDED USE This MMSAM ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MMSAM. This ELISA kit for research use only, not for therapeutic or diagnostic applications! PRINCIPLE OF THE ASSAY MMSAM ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MMSAM antibody and an MMSAM-HRP conjugate. The assay sample and buffer are incubated together with MMSAM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MMSAM concentration since MMSAM from samples and MMSAM-HRP conjugate compete for the anti-MMSAM antibody binding site. Since the number of sites is limited, as more sites are occupied by MMSAM from the sample, fewer sites are left to bind MMSAM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMSAM concentration in each sample is interpolated from this standard curve.

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