Human CYP4B1 ELISA Kit

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  • Alternative name

    Human CYPIVB1 ELISA Kit;Human cytochrome P450-HP ELISA Kit;Human P-450HP ELISA Kit;Human cytochrome P450 family 4 subfamily B member 1 ELISA Kit;Human cytochrome P450 4B1 ELISA Kit;Human cytochrome P450, family 4, subfamily B, polypeptide 1 ELISA Kit;Human cytochrome P450, subfamily IVB, polypeptide 1 ELISA Kit;Human microsomal monooxygenase ELISA Kit;

  • Catalog
    E011087
  • species
    Human
  • GeneCYP4B1
  • Standard CurveHuman CYP4B1 ELISA Kit
  • Other Species Mouse Cyp4b1 ELISA KitRabbit CYP4B1 ELISA KitRat Cyp4b1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CYP4B1. No significant cross-reactivity or interference between CYP4B1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CYP4B1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CYP4B1 ELISA Kit allows for the in vitro quantitative determination of CYP4B1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: CYP4B1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CYP4B1 antibody and an CYP4B1-HRP conjugate. The assay sample and buffer are incubated together with CYP4B1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CYP4B1 concentration since CYP4B1 from samples and CYP4B1-HRP conjugate compete for the anti-CYP4B1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CYP4B1 from the sample, fewer sites are left to bind CYP4B1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYP4B1 concentration in each sample is interpolated from this standard curve.
  • Human Cytochrome P450 4B1 Protein information
  • Uniprot ID CP4B1_HUMAN
  • Uniprot AC P13584; Q1HBI2; Q8TD85; Q8WWF2; Q8WWU9; Q8WWV0;
  • UniGene Hs.436317;
  • GeneID 1580
  • KEGG hsa:1580;
  • Human Cytochrome P450 4B1 Protein SEQUENCE
  • SEQUENCE 511 AA; 58991 MW; 91FED40949507EB8 CRC64;

    MVPSFLSLSF SSLGLWASGL ILVLGFLKLI HLLLRRQTLA KAMDKFPGPP

    THWLFGHALE IQETGSLDKV VSWAHQFPYA HPLWFGQFIG FLNIYEPDYA

    KAVYSRGDPK APDVYDFFLQ WIGRGLLVLE GPKWLQHRKL LTPGFHYDVL

    KPYVAVFTES TRIMLDKWEE KAREGKSFDI FCDVGHMALN TLMKCTFGRG

    DTGLGHRDSS YYLAVSDLTL LMQQRLVSFQ YHNDFIYWLT PHGRRFLRAC

    QVAHDHTDQV IRERKAALQD EKVRKKIQNR RHLDFLDILL GARDEDDIKL

    SDADLRAEVD TFMFEGHDTT TSGISWFLYC MALYPEHQHR CREEVREILG

    DQDFFQWDDL GKMTYLTMCI KESFRLYPPV PQVYRQLSKP VTFVDGRSLP

    AGSLISMHIY ALHRNSAVWP DPEVFDSLRF STENASKRHP FAFMPFSAGP

    RNCIGQQFAM SEMKVVTAMC LLRFEFSLDP SRLPIKMPQL VLRSKNGFHL

    HLKPLGPGSG K

  • UCSC uc001cqm.5; human. [P13584-1];


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