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Human SERT ELISA Kit

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  • Alternative name

    Sodium-dependent serotonin transporter ELISA KIT; 5HT transporter ELISA KIT; 5HTT ELISA KIT; Solute carrier family 6 member 4SLC6A4 ELISA KIT; HTT ELISA KIT; SERT ELISA KIT; 5HTT ELISA KIT
  • Catalog
    E034857
  • species
    Human
  • GeneSERT
  • Standard CurveHuman SERT ELISA Kit
  • Other Species Mouse SERT ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Serotonin Transporter (SERT). No significant cross-reactivity or interference between Serotonin Transporter (SERT) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 12.6pg/mL.
  • Intended UseHuman SERT ELISA Kit allows for the in vitro quantitative determination of SERT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serotonin Transporter (SERT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serotonin Transporter (SERT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serotonin Transporter (SERT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Serotonin Transporter (SERT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.


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