Intended UseMouse DPP IV ELISA Kit allows for the in vitro quantitative determination of DPP IV , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalProduct Information: DPP4 (mouse) ELISA Kit: Colorimetric Assay for Quantitative measurement of mouse DPP 4 in Serum, Plasma & Cell culture supernatants. Detection Range: 0.45 ng/ml - 100 ng/ml. 100 assays.
Background: Biobool's Mouse DPP IV ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse DPP IV. This assay employs an antibody specific for DPP IV coated on a 96-well plate. Standards and samples are pipetted into the wells and DPP IV present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse DPP IV antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DPP IV bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of DPP IV is typically less than 0.45 ng/ml. Detection Range: 0.45 ng/ml - 100 ng/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin, Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1alpha, MIP-1gamma, MIP-2, MIP-3beta, MIP-3alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Summary: Detection method- Absorbance (450 nm)
Species reactivity- Mouse
Application- Quantitative protein detection, establishing normal range, validation of antibody array results