Human Big ET ELISA Kit

Contact Us
  • Alternative name

    Endothelin-1 ELISA KIT; Preproendothelin-1 ELISA KIT; PPET1EDN1 ELISA KIT; PPET1 ELISA KIT; ET-1 ELISA KIT

  • Catalog
  • species
  • GeneBig ET
  • Standard CurveHuman Big ET ELISA Kit
  • Other Species Mouse Big ET ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of BEDN. No significant cross-reactivity or interference between BEDN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BEDN and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman Big ET ELISA Kit allows for the in vitro quantitative determination of Big ET , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Intended Uses: This BEDN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human BEDN. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||BEDN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BEDN antibody and an BEDN-HRP conjugate. The assay sample and buffer are incubated together with BEDN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BEDN concentration since BEDN from samples and BEDN-HRP conjugate compete for the anti-BEDN antibody binding site. Since the number of sites is limited, as more sites are occupied by BEDN from the sample, fewer sites are left to bind BEDN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BEDN concentration in each sample is interpolated from this standard curve.


Inquiry About Human Big ET ELISA Kit If you hope to order it or contact us directly, please contact us via We will get back to you in 12hrs,Thanks!