SpecificityThis assay has high sensitivity and excellent specificity for detection of Caspase 12. No significant cross-reactivity or interference between Caspase 12 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Caspase 12 and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseMouse Caspase-12 ELISA Kit allows for the in vitro quantitative determination of Caspase-12 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Categories/FamilyCell Biology
Product Description specificalPrinciple of the assay: Caspase 12 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Caspase 12 antibodies and an Caspase 12-HRP conjugate. The assay sample and buffer are incubated together with Caspase 12-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Caspase 12 concentration since Caspase 12 from samples and Caspase 12-HRP conjugate compete for the anti-Caspase 12 antibody binding site. Since the number of sites is limited, as more sites are occupied by Caspase 12 from the sample, fewer sites are left to bind Caspase 12-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Caspase 12 concentration in each sample is interpolated from this standard curve.