Human CD3D ELISA Kit

Alternative name
Human T-cell receptor T3 delta chain ELISA Kit;Human T3D ELISA Kit;Human CD3-DELTA ELISA Kit;Human IMD19 ELISA Kit;Human CD3d molecule ELISA Kit;Human T-cell surface glycoprotein CD3 delta chain ELISA Kit;Human CD3 antigen, delta subunit ELISA Kit;Human CD3 delta ELISA Kit;Human CD3d antigen, delta polypeptide (TiT3 complex) ELISA Kit;Human CD3d molecule, delta (CD3-TCR complex) ELISA Kit;Human OKT3, delta chain ELISA Kit;
Catalog
E007443
species
Human
GeneCD3D
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human CD3d. No significant cross-reactivity or interference between Human CD3d and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human CD3d and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.094ng/mL
Intended UseHuman CD3D ELISA Kit allows for the in vitro quantitative determination of CD3D , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CD3d in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CD3d. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human CD3d and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CD3d, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human CD3d. You can calculate the concentration of Human CD3d in the samples by comparing the OD of the samples to the standard curve.
Human T-cell surface glycoprotein CD3 delta chain Protein information
Uniprot ID CD3D_HUMAN
Uniprot AC P04234; A8MVP6;
UniGene Hs.504048;
GeneID 915
KEGG hsa:915;
Human T-cell surface glycoprotein CD3 delta chain Protein SEQUENCE
SEQUENCE 171 AA; 18930 MW; 6C1F248150186D21 CRC64;
MEHSTFLSGL VLATLLSQVS PFKIPIEELE DRVFVNCNTS ITWVEGTVGT
LLSDITRLDL GKRILDPRGI YRCNGTDIYK DKESTVQVHY RMCQSCVELD
PATVAGIIVT DVIATLLLAL GVFCFAGHET GRLSGAADTQ ALLRNDQVYQ
PLRDRDDAQY SHLGGNWARN K
UCSC uc001pss.2; human. [P04234-1];

Human CD3D ELISA Kit

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