Mouse Casp9 ELISA Kit

Alternative name
Mouse CASP-9 ELISA Kit;Mouse apoptotic protease Mch-6 ELISA Kit;Mouse apoptotic protease-activating factor 3 ELISA Kit;Mouse APAF-3 ELISA Kit;Mouse ICE-like apoptotic protease 6 ELISA Kit;Mouse ICE-LAP6 ELISA Kit;Mouse Mch6 ELISA Kit;Mouse AI115399 ELISA Kit;Mouse AW493809 ELISA Kit;Mouse caspase-9 ELISA Kit;Mouse caspase 9 ELISA Kit;
Catalog
E047244
species
Mouse
GeneCasp9
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse CASP9. No significant cross-reactivity or interference between Mouse CASP9 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse CASP9 and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.094ng/mL
Intended UseMouse Casp9 ELISA Kit allows for the in vitro quantitative determination of Casp9 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CASP9 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CASP9. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse CASP9 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CASP9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse CASP9. You can calculate the concentration of Mouse CASP9 in the samples by comparing the OD of the samples to the standard curve.
Mouse Caspase-9 subunit p10 Protein information
Uniprot ID CASP9_MOUSE
Uniprot AC Q8C3Q9;
UniGene Mm.88829;
GeneID 12371
KEGG mmu:12371;
Mouse Caspase-9 subunit p10 Protein SEQUENCE
SEQUENCE 454 AA; 49979 MW; 438A67EA66A6EE78 CRC64;
MDEADRQLLR RCRVRLVSEL QVAELWDALL SRELFTRDMI EDIQQAGSGS
RRDQARQLVT DLETRGRQAL PLFISCLEDT GQGTLASLLQ SGRQAAKQDP
EAVKPLDHLV PVVLGPMGLT AKEQRVVKLD PSQPAVGNLT PVVLGPEELW
PARLKPEVLR PETPRPVDIG SGGAHDVCVP GKIRGHADMA YTLDSDPCGH
CLIINNVNFC PSSGLGTRTG SNLDRDKLEH RFRWLRFMVE VKNDLTAKKM
VTALMEMAHR NHRALDCFVV VILSHGCQAS HLQFPGAVYG TDGCSVSIEK
IVNIFNGSGC PSLGGKPKLF FIQACGGEQK DHGFEVACTS SQGRTLDSDS
EPDAVPYQEG PRPLDQLDAV SSLPTPSDIL VSYSTFPGFV SWRDKKSGSW
YIETLDGILE QWARSEDLQS LLLRVANAVS AKGTYKQIPG CFNFLRKKLF
FKTS
UCSC uc008vpi.2; mouse.;

Category

Mouse Casp9 ELISA Kit