Human VTN ELISA Kit

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  • Alternative name

    Human VN ELISA Kit;Human S-protein ELISA Kit;Human Serum-spreading factor ELISA Kit;Human V75 ELISA Kit;Human VNT ELISA Kit;Human vitronectin ELISA Kit;Human complement S-protein ELISA Kit;Human epibolin ELISA Kit;Human serum spreading factor ELISA Kit;Human somatomedin B ELISA Kit;

  • Catalog
    E040382
  • species
    Human
  • GeneVTN
  • Other Species Mouse Vtn ELISA KitPorcine VTN ELISA KitRabbit VTN ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human VTN. No significant cross-reactivity or interference between human VTN and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human VTN is is typically less than 0.39 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the m
  • Intended UseHuman VTN ELISA Kit allows for the in vitro quantitative determination of VTN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VTN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VTN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VTN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VTN bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Somatomedin-B Protein information
  • Uniprot ID VTNC_HUMAN
  • Uniprot AC P04004; B2R7G0; P01141; Q9BSH7;
  • UniGene Hs.2257;
  • GeneID 7448
  • KEGG hsa:7448;
  • Human Somatomedin-B Protein SEQUENCE
  • SEQUENCE 478 AA; 54306 MW; 0D6DB5591CBFEF45 CRC64;

    MAPLRPLLIL ALLAWVALAD QESCKGRCTE GFNVDKKCQC DELCSYYQSC

    CTDYTAECKP QVTRGDVFTM PEDEYTVYDD GEEKNNATVH EQVGGPSLTS

    DLQAQSKGNP EQTPVLKPEE EAPAPEVGAS KPEGIDSRPE TLHPGRPQPP

    AEEELCSGKP FDAFTDLKNG SLFAFRGQYC YELDEKAVRP GYPKLIRDVW

    GIEGPIDAAF TRINCQGKTY LFKGSQYWRF EDGVLDPDYP RNISDGFDGI

    PDNVDAALAL PAHSYSGRER VYFFKGKQYW EYQFQHQPSQ EECEGSSLSA

    VFEHFAMMQR DSWEDIFELL FWGRTSAGTR QPQFISRDWH GVPGQVDAAM

    AGRIYISGMA PRPSLAKKQR FRHRNRKGYR SQRGHSRGRN QNSRRPSRAT

    WLSLFSSEES NLGANNYDDY RMDWLVPATC EPIQSVFFFS GDKYYRVNLR

    TRRVDTVDPP YPRSIAQYWL GCPAPGHL

  • UCSC uc002hbc.4; human.;


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