Human SPDEF ELISA Kit

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  • Alternative name

    Human prostate epithelium-specific Ets transcription factor ELISA Kit;Human prostate-specific Ets ELISA Kit;Human prostate-derived Ets factor ELISA Kit;Human PDEF ELISA Kit;Human PSE ELISA Kit;Human bA375E1.3 ELISA Kit;Human SAM pointed domain containing ETS transcription factor ELISA Kit;Human SAM pointed domain-containing Ets transcription factor ELISA Kit;

  • Catalog
    E034219
  • species
    Human
  • GeneSPDEF
  • Other Species Mouse Spdef ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human SPDEF. No significant cross-reactivity or interference between human SPDEF and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human SPDEF is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
  • Intended UseHuman SPDEF ELISA Kit allows for the in vitro quantitative determination of SPDEF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SPDEF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPDEF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPDEF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPDEF bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human SAM pointed domain-containing Ets transcription factor Protein information
  • Uniprot ID SPDEF_HUMAN
  • Uniprot AC O95238; B4DWH8; F5H778;
  • UniGene Hs.485158;
  • GeneID 25803
  • KEGG hsa:25803;
  • Human SAM pointed domain-containing Ets transcription factor Protein SEQUENCE
  • SEQUENCE 335 AA; 37518 MW; D3117E1AEEBA95EC CRC64;

    MGSASPGLSS VSPSHLLLPP DTVSRTGLEK AAAGAVGLER RDWSPSPPAT

    PEQGLSAFYL SYFDMLYPED SSWAAKAPGA SSREEPPEEP EQCPVIDSQA

    PAGSLDLVPG GLTLEEHSLE QVQSMVVGEV LKDIETACKL LNITADPMDW

    SPSNVQKWLL WTEHQYRLPP MGKAFQELAG KELCAMSEEQ FRQRSPLGGD

    VLHAHLDIWK SAAWMKERTS PGAIHYCAST SEESWTDSEV DSSCSGQPIH

    LWQFLKELLL KPHSYGRFIR WLNKEKGIFK IEDSAQVARL WGIRKNRPAM

    NYDKLSRSIR QYYKKGIIRK PDISQRLVYQ FVHPI

  • UCSC uc003ojq.3; human. [O95238-1];



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